T4 DNA Ligase - Discontinued


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T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5'-phosphate and the 3'-hydroxyl groups of adjacent nucleotides in either a blunt-ended or cohesive-ended configuration.

Features & Benefits

  • Catalyzes the joining of double-stranded DNA - for blunt or cohesive ends
  • No extras needed - supplied with 10x reaction buffer and ATP
  • Compatible with downstream applications - no loss of transformation efficiency

Applications

  • Ligation of cohesive and blunt-ended DNA fragments
  • Sealing nicks in double-stranded DNA
  • Ligation of synthetic linkers to blunt-ended DNA

Description

T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5'-phosphate and the 3'-hydroxyl groups of adjacent nucleotides in either a blunt-ended or cohesive-ended configuration. T4 DNA Ligase catalyses the joining of RNA to either DNA or RNA strands in a duplex molecule but will not join single-stranded nucleic acids. T4 DNA Ligase is ATP dependent.

Components

  • T4 DNA Ligase
  • 10x Reaction Buffer
  • ATP Solution

Concentration


5u/μl

Storage & Stability

T4 DNA Ligase can be stored for 12 months at -20°C. ATP solution should be aliquoted to avoid repeated freeze/thawing

Shipping conditions


On Blue Ice.






Other researchers use:


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