Tetro cDNA Synthesis Kit

Cat No. Size List Price* Qty  
BIO-65042 30 Reactions
$163.00
 
BIO-65043 100 Reactions
$433.00
 
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The Tetro cDNA Synthesis Kit contains all the necessary components to generate cDNA from an RNA template. The generated cDNA is suitable for PCR with gene-specific primers or for other downstream applications. The kit contains MMLV Reverse Transcriptase and is suitable for first strand cDNA synthesis, cDNA library construction and the production of templates for PCR amplification.

Features & Benefits

  • Highly sensitive -for high-quality, full length cDNA from as little as 10pg of total RNA
  • Ultra-stable reverse transcriptase -for long genes and rare transcripts
  • Simple -one-tube, one-step set-up for highly sensitive PCR
  • Broad dynamic range -10pg to 2μg of RNA

Applications

  • Construction of cDNA libraries
  • 2-step PCR assays
  • Generation of probes for hybridization
  • Gene cloning

Description

The Tetro cDNA Synthesis Kit contains our highly sensitive MMLV reverse transcriptase, oligo (dT)18 and random hexamer primers and all the necessary components to generate high quality cDNA from RNA templates (fig. 1). The first-strand cDNA generated is ideal for PCR (fig. 2) and can be used in a variety of other applications, such as  analyses of cellular RNAs, characterization of RNA splice variants, and the generation and cloning of cDNA. 

The Tetro cDNA Synthesis Kit is optimized for RT reactions over a wide range of total RNA concentrations (10pg-2μg), such that long and low-abundance cDNAs can be detected by amplification after cDNA synthesis. The kit contains oligo (dT)18 and random hexamer primers. The kit components are fully optimized to generate maximum yields of full-length cDNA.


Components

Reagent

30 reactions

100 reactions

5x RT Buffer

120µl

400µl

Reverse Transcriptase (200u/µl)

30µl

100µl

RNase Inhibitor (10u/µl)

30µl

100µl

dNTP Mix 10mM Total

30µl

100µl

Oligo (dT)18 Primer Mix

30µl

100µl

Random Hexamer Primer Mix

30µl

100µl

DEPC-treated Water

1.8ml

1.8ml


Storage & Stability

All kit components should be stored at -20°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.


Shipping conditions

Tetro cDNA Synthesis Kit is shipped on dry/blue ice


Certificates of Analysis (COAs)

Certificates of Analysis for Tetro cDNA Synthesis Kit are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 27 COAs for Tetro cDNA Synthesis Kit →




Customer Testimonials

We started to use this kit in our lab for all our routine cDNA synthesis and we haven't change since.
We find that the quality of the cDNA obtained is very good, the kit is very easy to use and it has a great quality-price ratio.
I definitely recommend it.

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