Tetro One-Step RT-PCR Kit - Discontinued


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Tetro One-Step RT-PCR Kit has been designed for easy and highly sensitive one-step RT-PCR using any RNA template. The kit contains a specially formulated enzyme blend, which includes IMMOLASE™ (our widely used hot-start DNA polymerase) and MMLV reverse transcriptase. This ensures highly efficient and sensitive transcription and PCR in one tube, without the need for optimization.

Features & Benefits

  • High-quality, full length cDNA from as little as 100pg of total RNA
  • Ultra-stable reverse transcriptase for long genes and rare transcripts
  • Simple one-tube, one-step set-up for highly sensitive RT-PCR
  • Broad dynamic range: 100pg to 2μg of RNA

Description

Tetro One-Step RT-PCR Kit has been designed for easy and highly sensitive one-step RT-PCR using any RNA template. The kit contains a specially formulated enzyme blend, which includes IMMOLASE™ (our widely used hot-start DNA polymerase) and MMLV reverse transcriptase. This ensures highly efficient and sensitive transcription and PCR in one tube, without the need for optimization. The Tetro One-Step RT-PCR Kit is ideal for the synthesis of double-stranded cDNA products for subsequent gene expression analysis, gene cloning, sequencing and virus detection etc.

The specially formulated enzyme blend ensures highly efficient and sensitive transcription from as little as 100pg up to 2μg of RNA. Even greater sensitivity and specificity can be achieved by the use of gene-specific primers, designed to match RNA/cDNA targets, maximizing amplification of these targets while minimizing amplification of other sequences. This can be further enhanced with the addition of DMSO (supplied) to remove problems with RNA secondary structure. The Kits also contain a potent RNase inhibitor, RiboSafe which protects RNA templates from degradation.


Components

Reagent

10 Reactions

25 Reactions

100 Reactions

Enzyme Mix

20µl

50µl

200µl

2x One-Step RT-PCR Reaction Buffer

250µl

625µl

2 x 1.25ml

RiboSafe RNase   Inhibitor (10u/µl)

10µl

25µl

100µl

50mM MgCl2 Solution

1.2ml

1.2ml

1.2ml

DMSO

1.2ml

1.2ml

1.2ml

DEPC-treated Water

1.2ml

1.2ml

1.2ml


Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.


Shipping conditions

On Dry Ice or Blue Ice.








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