Tetro Reverse Transcriptase

Cat No. Size List Price* Qty  
BIO-65050 10 000 Units
$276.00
 
BIO-65051 4 x 10,000u
$969.00
 
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Tetro Reverse Transcriptase is a highly sensitive, high stability MMLV reverse transcriptase. Tetro Reverse Transcriptase is optimized for reverse transcription reactions using a wide range of total RNA amounts, such that long and low abundance cDNAs can be detected by amplification after cDNA synthesis.

Tetro Reverse Transcriptase is suitable for first-strand cDNA synthesis, cDNA library construction, and the production of templates for RT-PCR analysis of gene expression.

Features & Benefits

  • Highly sensitive - for high-quality, full length cDNA from as little as 10pg of total RNA
  • Ultra-stable - for long genes and rare transcripts
  • High yield - produces high quality cDNA ideal for PCR
  • Broad dynamic range - 10pg to 2μg of RNA

Description

Tetro Reverse Transcriptase is a Moloney Murine Leukaemia Virus (MMLV) Reverse Transcriptase, which exhibits high stability, with no loss of activity following 1 week at room temperature. Tetro Reverse Transcriptase is highly sensitive even when the amount of template is a limiting factor (Fig. 1), with highly efficient and sensitive transcription, from as little as 10pg, up to 2μg of RNA (fig. 2).

Many RNA transcripts form stable secondary structures at lower temperatures, making them less suitable as templates for RT-PCR at those temperatures.

Tetro Reverse Transcriptase is suitable for first-strand cDNA synthesis, with total RNA, mRNA and in vitro transcribed RNA and shows excellent performance with gene-specific primers, Oligo (dT) as well as random hexamers, making it perfect for cDNA library construction and the production of templates for RT-PCR analysis of gene expression.


Components

Reagent

10,000 units

40,000 units

Tetro Reverse Transcriptase

50µl

4 x 50µl

5x Reaction Buffer

1.2ml

4 x 1.2ml


Concentration


200u/μl

Storage & Stability

All components should be stored at -20°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.


Shipping conditions

Shipped on dry/blue ice


Certificates of Analysis (COAs)

Certificates of Analysis for Tetro Reverse Transcriptase are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 12 COAs for Tetro Reverse Transcriptase →





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