Uracil DNA Glycosylase

Cat No. Size List Price* Qty  
BIO-27044 500 Units
$123.00
 
ADD TO CART
*For more information on pricing please contact us

Add to favorite products Email to a Friend Print View

Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single-stranded or double-stranded DNA, but not from RNA or oligonucleotides.

Features & Benefits

  • Removes uracils from DNA - causes fragmentation of DNA and stops amplification in a PCR
  • Easy-to-use - novel, sensitive mutant is irreversibly inactive after heating

Applications

  • Control of carry-over contamination in PCR
  • As a probe for protein-DNA interaction studies
  • Cloning of PCR products

Description

Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single-stranded or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases).

UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200mM).

Bioline UDG is purified to SDS-PAGE purity and is free of endonucleases, exonucleases, nickases and RNases.

Source: Recombinant E. coli strain carrying the over expressed gene of Uracil DNA Glycosylase from E. coli.


Components

Reagent

500 Units

Uracil DNA Glycosylase

500µl

10x Reaction Buffer

1.2ml


Concentration


1u/µl

Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.


Certificates of Analysis (COAs)

Certificates of Analysis for Uracil DNA Glycosylase are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 14 COAs for Uracil DNA Glycosylase →





Other researchers use:


^ Back to Top