Cat No. Size List Price* Qty  
BIO-21098 250 Units
BIO-21099 500 Units
*For more information on pricing please contact us

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VELOCITY DNA Polymerase is an ultra-fast thermostable enzyme possessing 3´-5´ proofreading exonuclease activity, which makes it ideal for high-fidelity PCR.

Features & Benefits

  • High-fidelity DNA polymerase - minimizing errors
  • High-processivity - for longer amplicons
  • Fast amplification - for short run times
  • Ideal for a wide range of templates - including long templates greater than 5kb


  • Cloning techniques where high fidelity is desirable
  • GC-rich templates
  • Blunt-end cloning
  • Site directed mutagenesis


VELOCITY delivers outstanding PCR yield with exceptional fidelity error rates of less than 4.4 x 10-7 bases, even from low template concentrations. High-processivity and extension rates result in shorter extension times in assays including those that are complex or contain impurities (Fig. 1).



250 Units

500 Units




5x Hi-Fi Buffer (contains 10mM Mg2+)

2 x 1.5ml

4 x 1.5ml

50mM MgCl2 Solution

1 x 1.2ml

1 x 1.2ml


1 x 1.25ml

1 x 1.25ml



Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

Shipping conditions

On Dry Ice or Blue Ice.

Certificates of Analysis (COAs)

Certificates of Analysis for VELOCITY DNA Polymerase are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 20 COAs for VELOCITY DNA Polymerase →

Customer Testimonials

"I did a side-by-side comparison of VELOCITY with Phusion. I got almost twice as much product with VELOCITY as from Phusion after cleanup and Nanodrop."
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"In comparison to other DNA polymerases on the market, Bioline’s are of the best quality at the most reasonable prices. Switching from our conventional high-fidelity enzyme to Bioline’s VELOCITY enzyme fixed one of the biggest problems I came across in my PhD project."

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