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HyperLadder FAQs

1.What are the optimal conditions for running my HyperLadder?
2.My HyperLadder doesn't look as it should when I run it, what should I do?
3.Do I need to pre-treat my HyperLadder prior to loading?
4.Are your ladders suitable for use in Polyacrylamide Gel Electrophoresis (PAGE)?
5.We do not wish to stain our gels using EtBr, will other stains still resolve the HyperLadder correctly?
6.Your conditions state that HyperLadder VI and MegaLadder 2Kb and 5Kb require pulse field electrophoresis in order to resolve all the bands correctly, what are the conditions required?
7.Do I need to add loading buffer to my Bioline marker to load it onto my gel?

1. What are the optimal conditions for running my HyperLadder?
Please see individual product insert (available online and included with each product) for the concentration of Agarose each ladder should be run on. The smaller the DNA product, the higher the percentage of Agarose required in the gel. All HyperLadders are ready-to-load, and require no addition of loading buffer prior to use.

2. My HyperLadder doesn't look as it should when I run it, what should I do?
Problems with our HyperLadders are extremely rare, so please make sure you have carried out each of the following correctly:
  • Used the correct percentage of Agarose in your gel for the specific marker in question.
  • Used 5µl of the marker per lane.
  • Used the correct concentration of stain (e.g. EtBr) to visualise correctly.
  • Vortexed the marker gently before use.
Should problems persist, please contact technical services for further assistance.

3. Do I need to pre-treat my HyperLadder prior to loading?
No pre-treatment, such as denaturing, is necessary prior to use.

4. Are your ladders suitable for use in Polyacrylamide Gel Electrophoresis (PAGE)?
Our HyperLadder range is designed for use on Agarose gels, and we do not recommended them for PAGE. However, should you wish to use Bioline HyperLadders in this technique, HyperLadder V, our small fragment DNA marker, would be the most appropriate.

5. We do not wish to stain our gels using EtBr, will other stains still resolve the HyperLadder correctly?
Other stains, such as SYBR® Green Gel Stain, can be used with our HyperLadders as effectively as EtBr. The fluorescence of the gel as a whole may vary depending on the stain used, however the relative intensities of the marker bands and of the samples run will remain the same, thus the HyperLadder remains quantitative..

6. Your conditions state that HyperLadder VI and MegaLadder 2Kb and 5Kb require pulse field electrophoresis in order to resolve all the bands correctly, what are the conditions required?
The conditions required are as follows:
0.3-0.5µg DNA, 0.5 TBE, 1% agarose, gel length 18cm. Forward Pulse 11V/cm, Reverse Pulse 1.5V/cm, 1sec.

7. Do I need to add loading buffer to my Bioline marker to load it onto my gel?
No, our Ladder range (excluding MegaLadder) is supplied in a ready-to-load format to be directly loaded onto your agarose gel. An additional vial of 5x Loading Buffer is supplied with each of our markers for use with your samples.

   

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