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Please see individual product insert (available online and included with each product) for the concentration of Agarose each ladder should be run on. The smaller the DNA product, the higher the percentage of Agarose required in the gel. All HyperLadders are ready-to-load, and require no addition of loading buffer prior to use.
Problems with our HyperLadders are extremely rare, so please make sure you have carried out each of the following correctly:
- Used the correct percentage of Agarose in your gel for the specific marker in question.
- Used 5µl of the marker per lane.
- Used the correct concentration of stain (e.g. EtBr) to visualise correctly.
- Vortexed the marker gently before use.
Should problems persist, please contact technical services for further assistance.
No pre-treatment, such as denaturing, is necessary prior to use.
Our HyperLadder range is designed for use on Agarose gels, and we do not recommended them for PAGE.
However, should you wish to use Bioline HyperLadders in this technique, HyperLadder V, our small fragment DNA marker, would be the most appropriate.
Other stains, such as SYBR® Green Gel Stain, can be used with our HyperLadders as effectively as EtBr. The fluorescence of the gel as a whole may vary depending on the stain used, however the relative intensities of the marker bands and of the samples run will remain the same, thus the HyperLadder remains quantitative..
The conditions required are as follows:
0.3-0.5µg DNA, 0.5 TBE, 1% agarose, gel length 18cm.
Forward Pulse 11V/cm, Reverse Pulse 1.5V/cm, 1sec.
No, our Ladder range (excluding MegaLadder) is supplied in a ready-to-load format to be directly loaded onto your agarose gel.
An additional vial of 5x Loading Buffer is supplied with each of our markers for use with your samples.
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