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Competent Cell Selection Table |
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Efficient DNA transformation of
competent cells is essential for successful cloning
applications. Bioline offers a wide range of E. coli host
strains to meet your requirements. We maintain rigorous
quality control standards to ensure lot-to-lot consistency
and the highest transformation efficiencies possible.
Each E. coli host has different characteristics, and for
optimal results, it is important to use the strain best
suited to your application.
The Bioline Competent Cell Selection Table
below provides a summary of the efficiencies, traits and
ideal applications for each Bioline Competent Cell strain. |
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Efficiency cfu/μg pUC19
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≥1 x 109 |
≥1 x 108 |
≥1 x 107 |
≥1 x 109 |
≥1 x 109 |
≥1 x 108 |
≥1 x 108 |
≥1 x 109 |
≥1 x 1010 |
≥1 x 108 |
≥1 x 109 |
≥1 x 107 |
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Competency
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C |
C |
C |
E |
C |
C |
C |
C |
E |
C |
C |
C |
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Strain Background |
K12 |
K12 |
K12 |
K12 |
K12 |
K12 |
K12 |
K12 |
K12 |
K12 |
K12 |
K12 |
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Blue white screening (lacZ) |
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Recombination deficient (recA) |
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Endonuclease deficient (endA)
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Restriction deficient (hsdRK-) |
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Methyl restriction deficient
(mcrA,
mcrB, mrr) |
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Single-strand ability (F'episome) |
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Phage resitance (fhuA, tonA
or T1R) |
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Unmethylated DNA (dam dcm) |
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