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PCR Enzyme Selection
  PCR Guide

Bioline produces one of the broadest portfolios of premium quality PCR Enzymes. The PCR Enzyme Selection Chart aims to simplify your polymerase selection process. Each polymerase has different characteristics and for optimal results, it is crucial to choose the enzyme that best suits your application. An overview of the main features of each Bioline polymerase is provided including information on available Ready-to-Use MasterMixes which contain polymerase, dNTPs, MgCl2 and additional additives.

For a more detailed overview of all our polymerases and available mastermixes, as well as a more extensive enzyme selection chart, please download our PCR enzyme guide.

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 Polymerase  Application  Ready-To-Use   Direct Gel Loading 
 Polymerases for Routine Applications
 BIOLASE  Highly sensitive PCR with high yields  BioMix
 BioMix Red
 BIOLASE Red
 BioMix Red
 MangoTaq  High throughput PCR  MangoMix  MangoTaq
 MangoMix
 Taq  Routine PCR  N/A  N/A
 Hot-Start Polymerases
 AccuSure  Hot-start PCR with high-fidelity  AccuSure Mix  N/A
 IMMOLASE  Hot-start PCR and quantitative assays  ImmoMix
 ImmoMix Red
 ImmoMix Red
 SAHARA  Low copy assays  SAHARA Mix  N/A
 High Fidelity Polymerases
 VELOCITY  High speed, high fidelity PCR assays   N/A  N/A
 ACCUZYME  Ultra-high fidelity PCR for subsequent cloning   ACCUZYME Mix  ACCUZYME Red
 AccuSure  Hot-start PCR with high-fidelity  AccuSure Mix  N/A
 BIO-X-ACT Short   Problematic PCR assays  BIO-X-ACT Short Mix   N/A
 BIO-X-ACT Long  Problematic long PCR assays  BIO-X-ACT Long Mix   N/A
 Polymerases for Special Applications
 BIO-X-ACT Short  Problematic PCR assays  BIO-X-ACT Short Mix  N/A
 BIO-X-ACT Long  Problematic long PCR assays  BIO-X-ACT Long Mix  N/A
 Diamond  Problematic PCR
 mis-matched primers not extended 
 Diamond Mix  N/A
 SAHARA  Low copy assays  SAHARA Mix  N/A
 

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