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PCR Enzyme Selection Table
Each polymerase has different characteristics, so it is crucial to choose the enzyme that suits your individual experiment. Bioline polymerases are available individually but some are also supplied as convenient ready-to-use 2x reaction mixes containing the polymerase of choice plus dNTPs, MgCI2 and further additives to achieve optimal reaction conditions.  
 
 
   Properties
   Template Length
Up to
5kb
Up to
5kb
Up to
10kb
Up to
10kb
Up to
5kb
Up to
25kb
Up to
8kb
Up to
5kb
Up to
5kb
Up to
5kb
   Hot-Start            
   Proofreading              
   High Processivity            
   Available Mixes
   Mixes    
   Applications
   Long Range PCR (over 10kb)                  
   High Specificity Assays            
   Blunt End Cloning                
   TA Cloning    
   GC-Rich Templates        
   Low Copy Templates        
   Site-Directed Mutagenesis                
   High-Fidelity PCR          
   Crude Sample PCR          
   Fast PCR      
   Direct Gel Loading            
   Colony PCR                

Recommended
Suitable


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