BIOLINE ::: GUIDES :::

PCR Enzyme Selection

PCR Guide

Bioline produces one of the broadest portfolios of premium quality PCR Enzymes. The PCR Enzyme Selection Chart aims to simplify your polymerase selection process. Each polymerase has different characteristics and for optimal results, it is crucial to choose the enzyme that best suits your application. An overview of the main features of each Bioline polymerase is provided including information on available Ready-to-Use MasterMixes which contain polymerase, dNTPs, MgCl₂ and additional additives.

For a more detailed overview of all our polymerases and available mastermixes, as well as a more extensive enzyme selection chart, please download our PCR enzyme guide.

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Polymerase	Application	Ready-To-Use	Direct Gel Loading
Polymerases for Routine Applications
BIOLASE	Highly sensitive PCR with high yields	BioMix BioMix Red	BIOLASE Red BioMix Red
MangoTaq	High throughput PCR	MangoMix	MangoTaq MangoMix
Taq	Routine PCR	N/A	N/A
Hot-Start Polymerases
AccuSure	Hot-start PCR with high-fidelity	AccuSure Mix	N/A
IMMOLASE	Hot-start PCR and quantitative assays	ImmoMix ImmoMix Red	ImmoMix Red
SAHARA	Low copy assays	SAHARA Mix	N/A
High Fidelity Polymerases
VELOCITY	High speed, high fidelity PCR assays	N/A	N/A
ACCUZYME	Ultra-high fidelity PCR for subsequent cloning	ACCUZYME Mix	ACCUZYME Red
AccuSure	Hot-start PCR with high-fidelity	AccuSure Mix	N/A
BIO-X-ACT Short	Problematic PCR assays	BIO-X-ACT Short Mix	N/A
BIO-X-ACT Long	Problematic long PCR assays	BIO-X-ACT Long Mix	N/A
Polymerases for Special Applications
BIO-X-ACT Short	Problematic PCR assays	BIO-X-ACT Short Mix	N/A
BIO-X-ACT Long	Problematic long PCR assays	BIO-X-ACT Long Mix	N/A
Diamond	Problematic PCR mis-matched primers not extended	Diamond Mix	N/A
SAHARA	Low copy assays	SAHARA Mix	N/A