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IPTG
  Cat No. Conc. Size Qty List Price* My Price  Ordering  
  BIO-37036 N/A 5g  1 $100.00 $100.00  
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Features



Applications

  • Blue/white color screening
  • Induction of lac operon for protein expression
  • Genes controlled by the lac or tac promotor/operator sequences are expressed to high levels in the presence of IPTG


Description

Isopropyl-ß-D-thiogalactopyranoside (IPTG) is a chemical analogue of galactose, which cannot be hydrolyzed by the enzyme ß--Galactosidase. Hence, it induces the E. coli lac operon activity by binding and inhibiting the lac repressor without being degraded. Genes controlled by the lac or tac promotor/operator sequences are expressed to high levels in the presence of IPTG.

IPTG is part of our Essentials range of products, which includes agaroses, buffers, PCR water and enhancers, antibiotic solutions, cloning reagents and protein digestion reagents.


Storage Conditions

IPTG powder can be stored for 12 months at –20°C.


Shipping Conditions

On Dry Ice or Blue Ice


Product Citations

1. Prabhakar, V., et al. FEBS Lett. 583(6), 983-991 (2009).
2. Chan, C-H., et al. Conserv. Gen. 9(4), 1067-1070 (2008).
3. Hamblin, K., et al. Mol. Microbiol. 68(6), 1395–1405 (2008).
4. Maruta, F., et al. J. Drug Targeting 15(4), 311-319 (2007).
5. Ross, P.J., et al. Infect. Immun. 72(3), 1568-1579 (2004).
6. Meng, G. and Fütterer, K. Nat. Struct. Biol. 10, 935-941 (2003).
7. Maruta, F., et al. Can. Gene Therapy 9(6), 543-552 (2002).


 
   
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