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| Cat No. |
Conc. |
Size |
Qty |
List Price* |
My Price |
Ordering |
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| BIO-21047S |
5u/µl |
25 Units |
1 |
$15.00
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$15.00
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| BIO-21046 |
5u/µl |
250 Units |
1 |
$130.00
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$130.00
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| BIO-21047 |
5u/µl |
500 Units |
1 |
$220.00
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$220.00
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| BIO-21048 |
5u/µl |
5000 Units |
1 |
$1,800.00
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$1,800.00
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Please contact
info@bioline.com for Bulk, Custom & OEM services.
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- Features
- Outstanding and robust performance
- For PCR assays requiring hot-start
- Excellent yield in quantitative assays
- Convenient set up at room temperature
- Leaves ´A´ overhang
- Available in ready-to-go versions (ImmoMix™ and ImmoMix™ Red)
- Applications
- Ultra-high specificity for multiplex reactions
- TA cloning
- Description
IMMOLASE™ is a heat-activated thermostable DNA polymerase isolated from a novel organism. IMMOLASE provides improved specificity when compared to standard polymerases and can eliminate the presence of non-specifics, such as primer-dimers and mis-primed products. IMMOLASE is inactive at room temperature and therefore, before primer extension, requires activation by heat treatment for 10 minutes. Subsequently, the reaction can be handled according to the user´s existing protocols for thermostable DNA polymerases.
The specificity and performance of IMMOLASE can be further improved with the use of 2x PolyMate Additive (not supplied, see associated products), which is designed for GC or AT-rich DNA, "dirty" templates or sequences with difficult melting profiles.
- Unit Definition
One unit will incorporate 10nmoles of dNTPs in 30min at
72°C.
- Concentration
5u/µl
- Storage Conditions
IMMOLASE can be stored for 12 months at –20°C.
- Product Citations
1. LaPensee, C.R., et al. Endocrinology 147(10), 4638-4645 (2006).
2. Rose, S.D., et al. Nucleic Acids Research 33(13), 4140-4156 (2005).
3. Thongnoppakhun, W., et al. BMC Medical Genetics 5(2) (2004).
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