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| Cat No. |
Conc. |
Size |
Qty |
List Price* |
My Price |
Ordering |
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| BIO-85034 |
N/A |
1ml (10 x 100µl) |
1 |
$99.00
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$99.00
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Please contact
info@bioline.com for Bulk, Custom & OEM services.
*Please login or
register to view and apply custom discounts.
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Add SOC to any Competent Cell Order for only $30*
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- Features
- High-level protein expression
- Protease deficient
- Transformation efficiency: ≥1 x 107 cfu/µg of pUC19
- Applications
- Regulation of T7 promoter expression
- Description
Especially engineered E.coli cells are often used to express a given construct and synthesize large amounts of the encoded protein. In such experiments the main points to consider are the type of promoter being used and the level of promoter control required.
BL21 Competent Cells, derivatives of E.coli Strain B, are suitable for high-level expression of a variety of recombinant proteins and are ideal hosts for the expression of proteins from vectors utilizing E.coli promoters. BL21 is a hardy strain which grows in minimal media and is deficient in key proteases.
BL21 (DE3) pLysE Competent Cells: This strain contains the T7 RNA polymerase gene as well as the plasmid pLysE that constitutively expresses T7 lysozyme, a natural inhibitor of T7 RNA polymerase. This strain is used to minimize basal level expression of potentially toxic gene products before induction with IPTG When induced with IPTG, the stronger T7 promotor overcomes the inhibition of T7 RNA polymerase expression by T7 lysozyme.
The pLysE plasmid provides the highest level of repression of the T7 RNA polymerasegene prior to induction.
Genotype: F- ompT hsdSB(rB-mB-) gal dcm (DE3) pLysE (CamR)
- Key Features
1.Marchenko, G.N., et al. Biochem J 372, 503-515 (2003).
2. Marani, M. et al. Mol. & Cell Biol. 22(11), 3577-3589 (2002).
*Only $30 if purchased in conjunction with any Competent cells.
This offer is available for a Limited Time Only.
This special offer cannot be used in conjunction with any other offer or discount.
Quote “SOCUS” on all orders placed by phone, fax or email.
This offer will automatically be applied to online orders. |
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