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| Cat No. |
Conc. |
Size |
Qty |
List Price* |
My Price |
Ordering |
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| BIO-85031 |
N/A |
1ml (10 x 100µl) |
1 |
$99.00
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$99.00
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Please contact
info@bioline.com for Bulk, Custom & OEM services.
*Please login or
register to view and apply custom discounts.
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Add SOC to any Competent Cell Order for only $30*
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- Features
- High-level protein expression
- Protease deficient
- Transformation efficiency: ≥1 x 107 cfu/µg of pUC19
- Applications
- Non-T7 promotor protein expression
- Description
Especially engineered E.coli cells are often used to express a given construct and synthesize large amounts of the encoded protein. In such experiments the main points to consider are the type of promoter being used and the level of promoter control required.
BL21 Competent Cells, derivatives of E. coli Strain B, are suitable for high-level expression of a variety of recombinant proteins and are ideal hosts for the expression of proteins from vectors utilizing E.coli promoters. BL21 is a hardy strain which grows in minimal media and is deficient in key proteases.
Bioline offers a series of BL21 Competent Cells for optimal protein expression and expression control.
This is an all purpose strain used for protein expression from vectors containing
E. coli promotors such as trc, tac, lPL and araD. (This strain lacks a T7 polymerase gene and can be used for non- T7 RNA polymerase protein expression systems. For T7 promotor driven protein expression, this strain requires infection with Lambda CE6 bacteriophage, which provides the T7 RNA polymerase.
Genotype: F- ompT hsdSB(rB-mB-) gal dcm
- Product Citations
1.Marchenko, G.N., et al. Biochem J 372, 503-515 (2003).
2. Marani, M. et al. Mol. & Cell Biol. 22(11), 3577-3589 (2002).
*Only $30 if purchased in conjunction with any Competent cells.
This offer is available for a Limited Time Only.
This special offer cannot be used in conjunction with any other offer or discount.
Quote “SOCUS” on all orders placed by phone, fax or email.
This offer will automatically be applied to online orders. |
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