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| Cat No. |
Conc. |
Size |
Qty |
List Price* |
My Price |
Ordering |
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| BIO-21044S |
1u/µl |
25 Units |
1 |
$10.00
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$10.00
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| BIO-21044 |
1u/µl |
500 Units |
1 |
$160.00
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$160.00
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| BIO-21045 |
1u/µl |
2500 Units |
1 |
$700.00
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$700.00
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Please contact
info@bioline.com for Bulk, Custom & OEM services.
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register to view and apply custom discounts.
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- Features
- Applications
- Routine PCR applications
- TA cloning
- High throughput
- Description
BIOLASE™ Red DNA polymerase is a formulation of our regular BIOLASE DNA polymerase, which contains a non-toxic and non-hazardous red dye. The red dye provides easy and quick identification of reactions to which the enzyme has been added, and facilitates the confirmation of complete mixing. When the reaction is complete, a sample of the reaction mix can be loaded directly onto the Agarose gel without the need for loading buffer, since the mix is of sufficiently high density to sink to the bottom of the gel. The red dye migrates towards the positive electrode, thereby providing a means to monitor the progress of the electrophoresis.
The presence of the dye has no effect on routine enzymatic manipulations, although rare exceptions may occur. In order to produce a reaction of sufficient density to allow for the direct loading of a sample onto a gel, we recommend using a minimum of 1.5u per 50µl reaction.
The specificity and performance of BIOLASE Red can be further improved with the use of 2x PolyMate Additive (not supplied, see associated products), which is designed for GC or AT-rich DNA, "dirty" templates or sequences with difficult melting profiles.
- Unit Definition
One unit will incorporate 10nmoles of dNTPs in 30min at
72°C.
- Concentration
1u/µl
- Storage Conditions
BIOLASE Red can be stored for 12 months at -20°C.
- Product Citations
1. Stark, B.C., et al. Journal Biotechnology Letters 25(12), 959-962 (2003).
2. Arndt, P.G., et al. The Journal of Immunology 175, 4049-4059 (2005)."
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