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| Cat No. |
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Qty |
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My Price |
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| BIO-21070 |
N/A |
500 Units |
1 |
$195.00
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$195.00
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Please contact
info@bioline.com for Bulk, Custom & OEM services.
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- Features
- Ideal for setting up new procedures
- Designed for easy optimizations of PCR applications
- Contains Ultra-pure dNTPs manufactured by Bioline
- Supplied with 2x PolyMate Additive for difficult or "dirty" templates
- Applications
- Routine PCR applications
- Products suitable for TA cloning
- Description
The BIOLASE™ PCR Kit contains all the necessary components to perform PCR assays on a wide range of DNA templates. In addition to dNTPs, the PCR Kit is based on our widely used BIOLASE DNA Polymerase, which achieves dependable results.
BIOLASE DNA polymerase is a highly purified thermostable DNA polymerase offering very high yield over a wide range of PCR templates, and is the ideal choice for most assays. BIOLASE™ is a robust preparation and consistently delivers high yields with minimal background. BIOLASE™ possesses 5´-3´ exonuclease activity and leaves an ´-A´- overhang such that the primer extension product is suitable for effective integration into TA cloning vectors.
The Kit Includes: 10x NH4 Buffer (Mg2+ free): Specially developed for BIOLASE™ to provide optimal reaction conditions.
50mM MgCl2 Solution For individual reaction optimization.
10mM dNTP Mix: Ultra-pure dNTPs are manufactured by Bioline and consist of a mix of dATP, dGTP, dCTP, and dTTP Lithium salts (pH 7.5).
2x PolyMate Additive: Provides an optimized composition of reagents, and is ideally suited to dirty/difficult templates with GC or AT-rich DNA, repetitive sequences or difficult melting profiles. PolyMate acts as a melting agent by allowing the DNA polymerase and oligonucleotides to have greater access to the template DNA.
- Kit Contents
- BIOLASE™ DNA Polymerase
- 10x NH4 Buffer
- 50mM MgCl2 Solution
- 10mM dNTP Mix
- 2x PolyMate Additive
- Concentration
5u/µl
- Storage Conditions
BIOLASE PCR Kit can be stored for 12 months at -20°C.
- Shipping Conditions
On Dry Ice or Blue Ice.
- Notes
BIOLASE is a trademark of Bioline.
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