 |
|
|
| Cat No. |
Conc. |
Size |
Qty |
List Price* |
My Price |
Ordering |
|
|
| BIO-21042S |
5u/µl |
25 Units |
1 |
$10.00
|
$10.00
|
 |
|
|
| BIO-21042 |
5u/µl |
500 Units |
1 |
$135.00
|
$135.00
|
|
|
|
| BIO-21043 |
5u/µl |
2500 Units |
1 |
$550.00
|
$550.00
|
|
|
|
| Bio-21066 |
5u/µl |
10 000 Units |
1 |
$1,850.00
|
$1,850.00
|
|
|
|
|
|
Please contact
info@bioline.com for Bulk, Custom & OEM services.
*Please login or
register to view and apply custom discounts.
|
|
|
- Features
- Consistent results
- Premium Taq polymerase suited to a wide range of applications
- Processes fragments of up to 5Kb
- Leaves ‘A’ overhang
- Available as ready-to-use 2x reaction mixes (BioMix™ Red)
- Applications
- Routine PCR applications
- Products suitable for TA cloning
- Description
BIOLASE™ is widely used by molecular biologists that have come to depend upon the robust performance of this reagent.
BIOLASE is a highly purified thermostable DNA polymerase offering very high yield over a wide range of PCR templates, and is the ideal choice for most assays. BIOLASE is a robust preparation and consistently delivers high yields with minimal background. BIOLASE possesses 5’-3’ exonuclease activity and leaves an ‘A’ overhang such that the PCR product is suitable for effective integration into TA cloning vectors.
BIOLASE is supplied with 10x NH4-based Reaction Buffer, which provides optimal conditions for most experiments. Additional MgCl2 is provided to allow reaction conditions to be adjusted to suit the template.
The specificity and performance of BIOLASE can be further improved with the use of 2x PolyMate Additive), which is designed for GC- or AT-rich DNA, “dirty” templates or sequences with a high level of secondary structure.
BIOLASE DNA Polymerase is purified from Thermus aquaticus.
- Unit Definition
One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.
- Concentration
5u/µl
- Storage Conditions
BIOLASE can be stored for 12 months at -20°C.
- Shipping Conditions
On Dry Ice or Blue Ice.
- Product Citations
1. Larsen, B., et al. Proc. Natl Acad. Sci. USA 97(4), 1683-1688 (2000).
2. Bennett, C.L., et al. The American Journal of Human Genetics 66, 461–468 (2000).
3. Tikhonova, E.B., et al. Journal of Bacteriology 184(23), 6499-6507 (2002).
|
|
| |
|
|
|
