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| Cat No. |
Conc. |
Size |
Qty |
List Price* |
My Price |
Ordering |
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| BIO-85037 |
N/A |
1ml (20 x 50µl) |
1 |
$129.00
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$129.00
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Please contact
info@bioline.com for Bulk, Custom & OEM services.
*Please login or
register to view and apply custom discounts.
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Add SOC to any Competent Cell Order for only $30*
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- Features
- Maintenance of F’ episome facilitated by antibiotic resistance, eliminating the need to select on minimal
media plates
- Available in two efficiencies: ≥108 or ≥109 cfu/μg
- Applications
- Single-stranded plasmid rescue
- Ideal strain for preparation of high-quality plasmid DNA
- Routine cloning, using λ DNA or plasmid vectors
- Blue/white color screening
- Description
BIOBlue Chemically Competent Cells provide an ideal host for optimal preparation of both high-quality plasmid and Lambda phage vectors. The BIOBlue strain allows blue/white color screening through α-complementation of the ß-galactosidase gene. The endA1 phenotype allows production of high-quality plasmid DNA. Single-stranded DNA can be produced from plasmids containing a phage f1 origin. BIOBlue is also an excellent host for M13 and related filamentous phage, and supports blue/white plaque screening and phage production. Maintenance of the F’ episome in this strain is facilitated via selection with tetracycline, unlike strains such as JM101 which require growth on minimal media. This strain is available in efficiencies of both >108 and >109 cfu/μg of pUC19.
- Genotype
recA1 ,em>endA1 gyrA96 thi-1 hsdR17(rk-, mk+) supE44 relA1 lac [F’ proAB lacIqZΔM15 Tn10(Tetr)]
- Storage Conditions
BIOBlue Chemically Competent Cells can be stored for 6 months at -80°C.
- Shipping Conditions
On dry ice
*Only $30 if purchased in conjunction with any Competent cells.
This offer is available for a Limited Time Only.
This special offer cannot be used in conjunction with any other offer or discount.
Quote “SOCUS” on all orders placed by phone, fax or email.
This offer will automatically be applied to online orders. |
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