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| Cat No. |
Conc. |
Size |
Qty |
List Price* |
My Price |
Ordering |
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| BIO-21049S |
4u/µl |
25 Units |
1 |
$20.00
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$20.00
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| BIO-21049 |
4u/µl |
250 Units |
1 |
$180.00
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$180.00
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| BIO-21050 |
4u/µl |
500 Units |
1 |
$320.00
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$320.00
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Please contact
info@bioline.com for Bulk, Custom & OEM services.
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register to view and apply custom discounts.
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- Features
- Ideal for long templates
- Higher fidelity than Taq due to proofreading activity
- Amplifies fragments up to 20Kb
- Available as a ready-to-use 2x reaction mix (BIO-X-ACT™ Long Mix)
- Applications
- For high fidelity PCR of long DNA fragments
- Suitable for TA cloning
- Description
BIO-X-ACT™ Long DNA Polymerase is a high-performance enzyme preparation specifically designed for difficult/problematic PCR applications requiring high processivity and fidelity. Two versions of BIO-X-ACT are available, for amplification of short and long DNA fragments respectively, and are the polymerases of choice for difficult applications that would normally fail with standard Taq polymerases.
BIO-X-ACT Long is recommended for long genomic DNA fragments of up to 20, or Lambda DNA fragments up to 30Kb.
For enhanced specificity, BIO-X-ACT Long is supplied with a vial of 5x Hi-Spec Additive.
Hi-Spec Additive is a very efficient enhancer, which helps to prevent the formation of false background bands and smearing.
- Unit Definition
One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.
- Concentration
4u/µl
- Storage Conditions
BIO-X-ACT Long DNA Polymerase can be stored for 12 months at -20°C.
- Shipping Conditions
- Product Citations
1. Eliahu, N., et al. Eukaryotic Cell 6(3), 421-429 (2007).
2. Wright, J.C., et al. Journal of Bacteriology 186(20), 6970-6982 (2004).
3. Shirak, A., et al. Genetics 174(3), 1573-1581 (2006).
- Notes
BIO-X-ACT is a trademark of Bioline.
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