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| Cat No. |
Conc. |
Size |
Qty |
List Price* |
My Price |
Ordering |
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| BIO-65044 |
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10 Reactions |
1 |
$55.00
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$55.00
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| BIO-65045 |
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25 Reactions |
1 |
$110.00
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$110.00
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| BIO-65046 |
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100 Reactions |
1 |
$345.00
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$345.00
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Please contact
info@bioline.com for Bulk, Custom & OEM services.
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register to view and apply custom discounts.
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- Features
- Simple to use one-step set-up
- Generate high-quality cDNA from as little as 100pg of total RNA
- Contains a unique blend of reverse transcriptase and hot-start polymerase to improve specificity
- Amplify longer genes and rare transcripts
- Unique 2x enzyme mix for the highest possible sensitivity
- Applications
- Quantitative PCR
- Gene expression analysis
- Gene cloning
- Virus detection
- Single-cell RT-PCR
- Description
The Tetro One-Step RT-PCR Kit has been designed for highly sensitive one-step RT-PCR reactions using any RNA template. The kit employs an enzyme formulation, which includes IMMOLASE™ (our widely used hot-start DNA polymerase) and MMLV reverse transcriptase.
The Tetro One-Step RT-PCR Kit provides highly specific reverse transcription and PCR in a single tube, using gene-specific primers on either total RNA or mRNA. The kit is provided with RNase inhibitor to protect template RNA from degradation. The proprietary buffer is highly optimized and balanced, leading to outstanding results. The kit is ideal for the synthesis of double-stranded cDNA products for subsequent cloning, sequencing, expression, or transcription analysis.
The kit can be used with starting amounts of RNA template from 100pg to 2µg. After cDNA synthesis has been performed, the reaction is heated to 95°C for 10 minutes to inactivate the reverse transcriptase, and simultaneously to activate the hot-start DNA polymerase IMMOLASE (included). IMMOLASE improves specificity by eliminating the presence of non-specifics, primer-dimers, and mis-primed products
- Storage Conditions
The Tetro One-Step RT-PCR Kit can be stored for 6 months at –20°C.
- Shipping Conditions
On Dry Ice or Blue Ice
- Notes
IMMOLASE is a trademark of Bioline
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