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| Cat No. |
Conc. |
Size |
Qty |
List Price* |
My Price |
Ordering |
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| BIO-85029 |
N/A |
2ml (10 x 200µl) |
1 |
$129.00
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$129.00
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Please contact
info@bioline.com for Bulk, Custom & OEM services.
*Please login or
register to view and apply custom discounts.
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Add SOC to any Competent Cell Order for only $30*
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- Features
- Bacteriophage T1-Resistant chemically competent cells
- Two efficiencies: ≥108 or ≥109 cfu/μg of DNA
- Accommodate larger plasmids
- Applications
- Blue/white color screening
- Construction of gene banks
- Generation of cDNA libraries using plasmid-derived vectors
- High quality plasmid preparation
- Hosting H13mp cloning vectors
- Description
α-Select Competent Cells contain a lacZ marker that provides α-complementation of the ß-galactosidase gene for blue/white color screening. The cells are ideal for generating cDNA libraries and subcloning. α-Select Competent Cells also provide recA1 and endA1 markers to minimize recombination and enhance the quality of plasmid DNA. pUC19 DNA is also provided as a positive control.
Both Silver and Gold Efficiency Chemically Competent Cells are available as bacteriophage T1-Resistant strains. Many laboratories have experienced bacteriophage T1 outbreaks, as T1 attacks E. coli and spreads rapidly. α-Select T1-Resistant cells protect samples from bacteriophage infection.
- Genotype
F- deoR endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) supE44 thi-1 phoA Δ(lacZYA-argF)U169 Φ80lacZΔM15 λ-
Bacteriophage T1-Resistant Chemically Competent Cells are available in two transformation efficiencies:
T1-Resistant Silver Efficiency ≥108 cfu/μg of pUC19
T1-Resistant Gold Efficiency ≥109 cfu/μg of pUC19 in convenient 50μl aliquots
- Storage Conditions
α-Select Competent Cells Bacteriophage T1-Resistant can be stored for 6 months at -80°C.
- Shipping Conditions
Dry Ice
*Only $30 if purchased in conjunction with any Competent cells.
This offer is available for a Limited Time Only.
This special offer cannot be used in conjunction with any other offer or discount.
Quote “SOCUS” on all orders placed by phone, fax or email.
This offer will automatically be applied to online orders. |
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