 |
|
|
| Cat No. |
Conc. |
Size |
Qty |
List Price* |
My Price |
Ordering |
|
|
| BIO-21069S |
2.5u/µl |
25 Units |
1 |
$20.00
|
$20.00
|
 |
|
|
| BIO-21068 |
2.5u/µl |
250 Units |
1 |
$150.00
|
$150.00
|
|
|
|
| BIO-21069 |
2.5u/µl |
500 Units |
1 |
$280.00
|
$280.00
|
|
|
|
|
|
Please contact
info@bioline.com for Bulk, Custom & OEM services.
*Please login or
register to view and apply custom discounts.
|
|
|
- Features
- High fidelity coupled with the specificity of hot-start
- Very high specificity and PCR sensitivity
- Convenient set up at room temperature
- Amplifies fragments up to 5Kb
- Available as a ready-to-use 2x reaction mix (AccuSure™ Mix)
- Applications
- PCR requiring high specificity combined with high-fidelity
- Blunt-end cloning
- Highly suited to site directed mutagenesis
- Description
AccuSure™ is a heat-activated enzyme possessing 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities, offering extremely high fidelity and enhanced specificity. AccuSure is ideally suited for difficult PCR templates, such as DNA secondary structures and microsatellites. AccuSure produces blunt-ended amplicons of up to 5Kb in length
AccuSure is optimized for use in a detergent-free system, making it suitable for direct loading of samples on to DHPLC columns.
AccuSure is supplied with 10x reaction buffer containing MgSO4, which provides optimal final reaction conditions (1.5mM Mg2+) for most experiments. In order to allow optimization of reaction conditions, additional MgCl2 is provided.
The specificity and performance of AccuSure can be further improved with the use of 2x PolyMate Additive, which is designed for GC‐ or AT-rich DNA, "dirty" templates or sequences with a high level of secondary structure.
- Unit Definition
One unit will incorporate 10nmoles of dNTPs in 30min at
72°C.
- Concentration
2.5u/µl
- Storage Conditions
AccuSure can be stored for 12 months at -20°C.
Note: Purchase of this product does not convey a licence to perform any patented process.
|
|
| |
|
|
|
