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| Cat No. |
Conc. |
Size |
Qty |
List Price* |
My Price |
Ordering |
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| BIO-21103 |
10x |
50 Reactions |
1 |
$295.00
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$295.00
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- Features
- Fast addition of 3’-A overhang to blunt ended DNA
- Uses exonuclease inhibitor so no post PCR purification required
- Applications
- TA cloning techniques where high fidelity is desirable
- Description
Proofreading DNA Polymerases such as VELOCITY provide high fidelity, however the resulting PCR product is blunt-ended. This is due to the 3’→5’ exonuclease activity which removes the 3’-A overhangs normally added by Taq DNA polymerase. These 3’-A overhangs are very useful for efficient cloning of these fragments into suitable plasmids (TA cloning), and effectively prevent insert-to-insert ligation, eliminating possible tandem inserts.
The PCR Tailing Mix uses Taq DNA polymerase and an exonuclease inhibitor to add a single terminal A, whilst inhibiting the 3’→5’ exonuclease activity of the proofreading DNA polymerase.
- Concentration
5x
- Storage Conditions
PCR Tailing Mix can be stored for 12 months at –20°C.
- Shipping Conditions
On Dry Ice or Blue Ice
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