 |
|
|
| Cat No. |
Conc. |
Size |
Qty |
List Price* |
My Price |
Ordering |
|
|
| BIO-21104 |
N/A |
250 Units |
1 |
$190.00
|
$190.00
|
 |
|
|
|
|
Please contact
info@bioline.com for Bulk, Custom & OEM services.
*Please login or
register to view and apply custom discounts.
|
|
|
- Features
- High-speed, high fidelity DNA polymerase
- Robust performance with problematic GC and AT rich targets
- Long Range PCR of complex genomic DNA (up to 10kb)
- Robust, requires minimal optimization of the reaction
- New PCR Tailing Mix to improve the efficiency of cloning
- Applications
- Cloning techniques where high fidelity is desirable
- Description
To enhance the cloning efficiency of DNA amplified with VELOCITY, Bioline has developed a new VELOCITY PCR Kit. The kit contains VELOCITY DNA polymerase to generate error free PCR products and a PCR Tailing Mix to add a 3’-A overhang, allowing TA cloning.
VELOCITY PCR products are blunt-ended due to the 3’→5’ exonuclease activity of the polymerase which removes 3’-A overhangs. A 3’-A overhang is useful, however, as it facilitates more efficient cloning into plasmid vectors and help prevent insert-to-insert ligation, eliminating possible tandem inserts. In order to generate a 3’-A overhang, the kit also contains a PCR Tailing Mix. This uses a uniquely blended Taq polymerase to add a single Adenine base as well as an exonuclease inhibitor to reduce the 3’→5’ exonuclease activity of the VELOCITY, thus eliminating the need for purification of the PCR products prior to the addition of the overhang.
The result is ‘sticky ended’ PCR fragments that can be efficiently ligated straight into a TA cloning vector and transformed directly into competent cells (such as Bioline’s Alpha-Select Competent Cells) without further cleaning or purification.
- Size
The VELOCITY PCR Kit contain enough VELOCITY DNA Polymerase for optimization of conditions and 20 tailing reactions.
- Storage Conditions
VELOCITY PCR Kit can be stored for 12 months at –20°C.
- Shipping Conditions
On Dry Ice or Blue Ice
|
|
| |
|
|
|
