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| Cat No. |
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Size |
Qty |
List Price* |
My Price |
Ordering |
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| BIO-21072 |
20u/µl |
50 Reactions |
1 |
$175.00
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$175.00
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- Features
- Fast and efficient RNA transcription from any DNA template containing a T7 promotor
- Convenient, reliable, cost-effective
- Contains all the necessary reagents for T7 transcription reactions
- Applications
- Production of RNA and double-stranded RNA for siRNA experiments
- Generation of specific radioactive RNA probes
- Synthesis of RNA for RNA-protein interaction, splicing and processing studies
- RNA production for in vitro translation
- Description
The T7 Transcription Kit contains all the necessary components to carry
out fast and efficient transcription of RNA from any DNA with a T7 promotor
sequence (Fig. 1). It can also be used to produce RNA from cloned inserts.
The T7 RNA polymerase will incorporate labeled NTPs, and products of this
enzyme can be used as probes for screening purposes. The kit contains
Bioline´s RiboSafe RNase inhibitor and Ultimate Reaction Buffer, a proprietary
buffer specially formulated and optimized to give exceptional performance and
reliability.
The T7 Transcription Kit contains enough reagents for either 50 x 20μl reactions
of high yield RNA transcription, or 1 x 1ml.
- Storage Conditions
T7 RNA Polymerase can be stored for 12 months at -20°C. DTT should be
aliquotted to avoid instability due to multiple freeze/thaw cycles.
- Product Citations
1. Jamie-Lee Berry, J-L. and Bulleid, N.J. Biosci. Horizons 2(1),13-21 (2009).
2. Fen, C.X., et al. J. Mol. Biol. 371(5), 1238-1248 (2007).
3. Dukes, J.P., et al. Archives Virol. 151(6), 1093-1106 (2006).
4. Zheleznaya, L.A. et al. Anal. Biochem. 34(1),123-126 (2006).
- Notes
The T7 gene expression system is licensed under U.S. Patent Nos. 4,952,496; 5,693,489 and 5,869,320.
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