Bioline Home Page    
A Meridian Life Science® Company
Your Country
   Login | Shopping Cart
 
 
T4 DNA Ligase
  Cat No. Conc. Size Qty List Price* My Price  Ordering  
  BIO-27026 5u/µl 500u  1 $110.00 $110.00  
Please contact info@bioline.com for Bulk, Custom & OEM services.
*Please login or register to view and apply custom discounts.

Features

  • Catalyzes the joining of double-stranded DNA
  • Supplied with 10x reaction buffer and ATP
  • No loss of transformation efficiency


Applications

  • Ligation of cohesive and blunt-ended DNA fragments
  • Sealing nicks in double-stranded DNA
  • Ligation of synthetic linkers to blunt-ended DNA


Description

T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5'-phosphate and the 3'-hydroxyl groups of adjacent nucleotides in either a blunt-ended or cohesive-ended configuration. T4 DNA Ligase catalyses the joining of RNA to either DNA or RNA strands in a duplex molecule but will not join single-stranded nucleic acids. T4 DNA Ligase is ATP dependent.


Concentration

10u/μl


Storage Conditions

T4 DNA Ligase can be stored for 12 months at -20°C. ATP solution should be aliquoted to avoid repeated freeze/thawing.


Shipping Conditions

On Blue Ice


Product Citations

1. Lopez, M., et al. Am. J. Enol. Vitic. 60(2), 215-222 (2009).
2. An, J., et al. Conserv. Genet. 5(1), 121-125 (2004).


 
   
Copyright ©2010 Bioline