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| Cat No. |
Conc. |
Size |
Qty |
List Price* |
My Price |
Ordering |
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| QT285-01 |
2x |
25 Reactions |
1 |
$70.00
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$70.00
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| QT285-02 |
2x |
250 Reactions |
1 |
$535.00
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$535.00
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| QT285-05 |
2x |
500 Reactions |
1 |
$975.00
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$975.00
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| QT285-20 |
2x |
2000 Reactions |
1 |
$3,350.00
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$3,350.00
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- Features and Benefits
- Flexible: RNA templates including viral RNA, poly(A) RNA or total RNA may be employed
- Reproducible: master-mix contains reverse transcriptase for efficient cDNA synthesis together with an optimized amount of highly engineered hot-start SensiTaq enzyme, to provide reproducible real-time data
- Sensitive: accurate linear standard curve from a 10-fold RNA dilution series over a broad dynamic range
- Convenient: reduced pipetting steps and possible contamination and RNA degradation
- Instrument Compatibility
ABI 7500 and StepOne™, Stratagene Mx4000®, Mx3000P® and Mx3005P®. (See product selection table).
- Description
The SensiMix SYBR Low-ROX Kit contains SYBR® Green I dye and a low concentration of ROX fluorescent dye, making it specifically suitable for use on real-time instruments that measure this passive reference signal. Such instruments include the ABI 7500, StepOne™, and the Stratagene Mx3000P® and Mx3005P® platforms. The master-mix also contains reverse transcriptase for efficient cDNA synthesis together with an optimized amount of highly engineered hotstart SensiTaq enzyme, allowing highly reproducible first strand cDNA synthesis and subsequent real-time PCR assays in one tube.
- Volume
QT285-01: 25 x 50µl Reactions: 1 x 625µl
QT285-02: 250 x 50µl Reactions: 5 x 1.25ml
QT285-05: 500 x 50µl Reactions: 10 x 1.25ml
QT285-20: 2000 x 50µl Reactions: 40 x 1.25ml
- Components
2x Reaction mix consisting of:
- Heat-activated DNA polymerase
- Sensi reverse transcriptase
- Internal reference
- Ultra-pure dNTPs
- MgCl2 (6mM)
- SYBR® Green I
Plus separate vials of:
- 50mM MgCl2
- RNase inhibitor
- DEPC-treated water
- Kit Size
The kit size is based on a 50µl final reaction volume.
- Storage Conditions
6 months at -20°C. Avoid exposure of the SYBR® Green I to light.
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