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| Cat No. |
Conc. |
Size |
Qty |
List Price* |
My Price |
Ordering |
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| QT605-02 |
2x |
250 Reactions |
1 |
$290.00
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$290.00
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| QT605-05 |
2x |
500 Reactions |
1 |
$535.00
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$535.00
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| QT605-20 |
2x |
2000 Reactions |
1 |
$1,925.00
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$1,925.00
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Please contact
info@bioline.com for Bulk, Custom & OEM services.
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register to view and apply custom discounts.
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- Features and Benefits
- Simple and reproducible: a choice of optimized kits for different platforms, just add primers and template, reducing possible errors in set-up
- Specific and sensitive: for detection of a wide range of template concentration, even from hard to obtain and low copy number samples
- Fast protocols: can be used with the new generation of fast cyclers without compromising performance
- Flexible: validated with all major real-time instruments
- 2x master mix: premixed with SYBR Green I and an internal reference for convenience
- Instrument Compatibility
ABI 7000, 7300, 7700, 7900, 7900HT and StepOne™. (See product selection table)
- Description
Versatility
- Mix optimised for SYBR® Green I chemistry.
- Suitable for high throughput applications e.g. target validation for drug discovery.
- Designed to work across a range of block and rotor-based instruments.
SensiMix SYBR kit contains all the components necessary to perform consistent and reproducible real-time PCR assays with your DNA template. The kits deliver highly specific and sensitive results across a wide dynamic range of template concentrations (over 10 fold), producing reliable real-time data quickly and easily. The kits are suitable for DNA templates or RNA templates following reverse transcription with the Bioline cDNA Synthesis Kit (BIO-65025) and are compatible with the ABI 7000, 7700, 7900HT and StepOne™ platforms. (See product selection table)
The kits are powered by SensiTaq, a highly-sensitive hot-start DNA polymerase that provides higher specificity, and ROX passive reference fluorescent dye, making it specifically suitable for use on real-time instruments that measure this passive reference signal.
- Volume
QT605-02: 250 x 50µl Reactions: 5 x 1.25ml
QT605-05: 500 x 50µl Reactions: 10 x 1.25ml
QT605-20: 2000 x 50µl Reactions: 40 x 1.25ml
- Components
2x Reaction mix consisting of:
- Heat-activated DNA polymerase
- Ultra-pure dNTPs
- MgCl2 (6mM)
- Internal reference
- SYBR® Green I
- Kit Size
The kit size is based on a 50µl final reaction volume.
- Storage Conditions
6 months at -20°C. Avoid exposure of the SYBR® Green I to light.
- Product Citations
1. Lappas M., et al. Placenta 30(3): 256-262 (2009)
2. Rosker C., et al. J. Biol. Chem. 284(8): 5186-5194 (2009)
3. Barker H.E, et al. BMC Developmental Biology 8(1): 94 (2008)
4. Johnson C.E. et al. StemCells 25: 1913–1923 (2007)
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- “What was a problem before now works perfectly! I tried several SYBR Green Kits but SensiMix SYBR worked best. Triplicates have very good standard deviations and detection of even low transcript levels via real-time RT-PCR are no problem anymore in Plasmodium falciparum.”
Dipl. biol. Anne Röseler,
Nutritional Biochemistry,
Justus-Liebig University Giessen,
Germany.
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