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| Cat No. |
Conc. |
Size |
Qty |
List Price* |
My Price |
Ordering |
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| QT615-02 |
2x |
250 Reactions |
1 |
$290.00
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$290.00
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| QT615-05 |
2x |
500 Reactions |
1 |
$535.00
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$535.00
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| QT615-20 |
2x |
2000 Reactions |
1 |
$1,925.00
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$1,925.00
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Please contact
info@bioline.com for Bulk, Custom & OEM services.
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register to view and apply custom discounts.
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- Features and Benefits
- Simple and reproducible: premixed with SYBR Green I and fluorescein, just add primers and template, reducing errors in set-up
- Specific and sensitive: for detection of a wide range of template concentration, even from hard to obtain and low copy number samples
- Fast protocols: can be used with the new generation of fast cyclers without compromising performance
- Flexible: suitable for block-based instruments where fluorescein is required to allow calculation of dynamic well factors
- Instrument Compatibility
Bio-Rad iCycler®, iQTM5 and MyiQ™. (See product selection table)
- Description
- The SensiMix SYBR & Fluorescein Kit contains fluorescein, making it specifically suitable for use on real-time instruments that measure fluorescein passive signal, such as the Bio-Rad iCycler®, iQ™5 and MyiQ™ platforms. Both SYBR® Green I and fluorescein are premixed for immediate use.
With SYBR® Green I and fluorescein in their premixed and optimized concentrations, as few as 10 copies of a target gene can be detected
- Volume
QT615-02: 250 x 50µl Reactions: 5 x 1.25ml
QT615-05: 500 x 50µl Reactions: 10 x 1.25ml
QT615-20: 2000 x 50µl Reactions: 40 x 1.25ml
- Components
2x Reaction mix consisting of:
- Heat-activated DNA polymerase
- Ultra-pure dNTPs
- MgCl2 (6mM)
- Fluorescein
- SYBR® Green I
Plus separate vials of:
- Kit Size
The kit size is based on a 50µl final reaction volume.
- Storage Conditions
6 months at -20°C. Avoid exposure of the SYBR® Green I to light.
- Product Citations
1. Dhawan R. et al. The Plant Cell 21: 1000-1019 (2009)
2. Maatouk D.M. et al. Human Molecular Genetics 17(19): 2949-2955 (2008)
3. Del Rosario B.C. & Pemberton L.F. Molecular and Cellular Biology 28(7): 2113-2124 (2008)
4. Miller M.R. et al. J. Nutr. 138(11):2179-2185 (2008)
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