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| Cat No. |
Conc. |
Size |
Qty |
List Price* |
My Price |
Ordering |
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| QT725-01 |
2x |
25 Reactions |
1 |
$70.00
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$70.00
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| QT725-02 |
2x |
250 Reactions |
1 |
$535.00
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$535.00
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| QT725-05 |
2x |
500 Reactions |
1 |
$975.00
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$975.00
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| QT725-20 |
2x |
2000 Reactions |
1 |
$3,350.00
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$3,350.00
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Please contact
info@bioline.com for Bulk, Custom & OEM services.
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register to view and apply custom discounts.
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- Features and Benefits
- Reproducible: master-mix contains reverse transcriptase for efficient cDNA synthesis together with an optimized amount of highly engineered hot-start SensiTaq enzyme, to provide reproducible real-time data
- Sensitive: accurate quantification of any RNA from low copy targets. (i.e., pathogen detections)
- Convenient: reduced pipetting steps and possible contamination and RNA degradation
- Instrument Compatibility
ABI 7000, 7300, 7500, 7700, 7900, 7900HT, StepOneTM, StepOneTM Plus, Roche LightCycler® 480, Bio-Rad iCycler®, iQTM5 and MyiQTM, OpticonTM, OpticonTM2, Chromo4TM, MiniOpticonTM, CFX96TM, CFX384TM, Cepheid SmartCycler®, Corbett Rotor-GeneTM 3000, Rotor-GeneTM 6000, Eppendorf Mastercycler® ep realplex, Techne Quantica®, Stratagene Mx4000®, Mx3000P® and Mx3005P®. (See product selection table).
- Description
The SensiMix Probe One-Step Kit provides optimal performance and maximum convenience for real-time, quantitative analysis of RNA templates in a single reaction format on real-time instruments such as the Corbett Research Rotor-Gene™ 3000 and Roche LightCycler® 480. A separate tube of ROX fluorescent dye is supplied as an option to measure this passive reference signal on real-time instruments such as the ABI 7500, 7900HT and the Stratagene Mx3000P® and Mx3005P® platforms. (See product selection table).
The SensiMix Probe One-Step Kit has been optimized for use on a wide variety of probe chemistries including TaqMan® probes, molecular beacons, scorpions and FRET probes. Applications include real-time PCR and allelic discriminations
- Volume
QT725-01: 25 x 50µl Reactions: 1 x 625µl
QT725-02: 250 x 50µl Reactions: 5 x 1.25ml
QT725-05: 500 x 50µl Reactions: 10 x 1.25ml
QT725-20: 2000 x 50µl Reactions: 40 x 1.25ml
- Components
2x Reaction mix consisting of:
- Heat-activated DNA polymerase
- Sensi reverse transcriptase
- Internal reference
- Ultra-pure dNTPs
- MgCl2 (6mM)
Plus separate vials of:
- 50mM MgCl2
- RNase inhibitor
- DEPC-treated water
- ROX Internal reference 25µM
- Kit Size
The kit size is based on a 50µl final reaction volume.
- Storage Conditions
6 months at -20°C. Avoid exposure of the SYBR® Green I to light.
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