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SensiMix Probe Kit
  Cat No. Conc. Size Qty List Price* My Price  Ordering  
  QT705-02 2x 250 Reactions  1 $290.00 $290.00  
  QT705-05 2x 500 Reactions  1 $535.00 $535.00  
  QT705-20 2x 2000 Reactions  1 $1,925.00 $1,925.00  
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Features and Benefits

  • Simple: just add fluorescent detection chemistry, primers, and template

  • Specific and sensitive: for detection of a wide range of template concentration, even from hard to obtain and low copy number samples

  • Fast protocols: can be used with the new generation of fast cyclers without compromising performance

  • Flexible: ideal for multiplex assays


Instrument Compatibility

ABI 7000, 7300, 7500, 7700, 7900, 7900HT, StepOneTM, StepOneTM Plus, Roche LightCycler® 480, Bio-Rad iCycler®, iQTM5 and MyiQTM, OpticonTM, OpticonTM2, Chromo4TM, MiniOpticonTM, CFX96TM, CFX384TM, Cepheid SmartCycler®, Corbett Rotor-GeneTM 3000, Rotor-GeneTM 6000, Eppendorf Mastercycler® ep realplex, Techne Quantica®, Stratagene Mx4000®, Mx3000P® and Mx3005P® (See product selection table).


Description

The SensiMix Probe Kit is a high-performance product with superior sensitivity and specificity on a wide range of real-time instruments such as the Corbett Research Rotor-Gene™ 3000 and Roche LightCycler® 480. A separate tube of ROX fluorescent dye is supplied in order to facilitate the option of measuring this passive reference signal on real-time instruments including the ABI 7500 and 7900HT and Stratagene Mx3000P® and Mx3005P® platforms.

The SensiMix Probe Kit has been designed for fast, quantitative real-time PCR using sequence-specific probes in DNA assays. The kit has been optimized for use on a wide variety of assays, such as routine real-time PCR, allelic discrimination assays, and probe chemistries including TaqMan®, molecular beacons, Scorpions® and FRET probes


Volume

QT705-02: 250 x 50µl Reactions: 5 x 1.25ml
QT705-05: 500 x 50µl Reactions: 10 x 1.25ml
QT705-20: 2000 x 50µl Reactions: 40 x 1.25ml


Components

2x Reaction mix consisting of:

  • Heat-activated DNA polymerase
  • Ultra-pure dNTPs
  • MgCl2 (6mM)


Plus separate vials of:

  • 50mM MgCl2
  • 25mM ROX Internal reference


Kit Size

The kit size is based on a 50µl final reaction volume.


Storage Conditions

6 months at -20°C. Avoid exposure of the SYBR® Green I to light.


 
   
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