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| Cat No. |
Conc. |
Size |
Qty |
List Price* |
My Price |
Ordering |
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| BIO-21089S |
4u/µl |
25 Units |
1 |
$15.00
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$15.00
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| BIO-21088 |
4u/µl |
250 Units |
1 |
$135.00
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$135.00
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| BIO-21089 |
4u/µl |
500 Units |
1 |
$250.00
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$250.00
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| BIO-21090 |
4u/µl |
2500 Units |
1 |
$1,050.00
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$1,050.00
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Please contact
info@bioline.com for Bulk, Custom & OEM services.
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register to view and apply custom discounts.
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- Features
- New Optimized Protocol
- Highly suited to low copy assays
- Heat activation for ultra-high specificity
- High yield coupled with high fidelity
- Convenient set up at room temperature
- Amplifies fragments up to 5Kb
- Applications
- Ideal for low copy PCR assays
- Suitable both for TA cloning
- Description
SAHARA™ DNA Polymerase is a heat-activated high-performance preparation of enzymes specifically designed for low-copy or challenging PCR assays which require both high processivity and high fidelity. SAHARA provides improved specificity and very high PCR sensitivity, thereby eliminating the presence of non-specifics such as primer-dimers and mis-primed products. SAHARA is inactive at room temperature and therefore requires activation by heat treatment for 10 minutes at 95°C prior to the PCR reaction being performed.
SAHARA DNA Polymerase is recommended for short genomic DNA fragments of up to 3Kb, or up to 5Kb on Lambda DNA. SAHARA is ideal for direct cloning without the need to verify the sequence prior to expression.
The specificity and performance of SAHARA DNA Polymerase can be further improved with the use of 2x PolyMate Additive, which is designed for GC- or AT-rich DNA, "dirty" templates or sequences with a high level of secondary structure.
- Unit Definition
One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.
- Concentration
4u/µl
- Storage Conditions
SAHARA can be stored for 12 months at –20°C.
- Shipping Conditions
On Dry Ice or Blue Ice
- Notes
SAHARA is a trademark of Bioline.
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