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| Cat No. |
Conc. |
Size |
Qty |
List Price* |
My Price |
Ordering |
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| BIO-27028S |
N/A |
5 Reactions |
1 |
$10.00
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$10.00
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| BIO-27027 |
N/A |
50 Reactions |
1 |
$75.00
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$75.00
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| BIO-27028 |
N/A |
100 Reactions |
1 |
$140.00
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$140.00
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- Features
- Dramatically decreases time for DNA cloning
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- Rapid 5 to 15 minute protocol at room temperature
- Efficient and reliable ligations of cohesive and
blunt-ended DNA
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- No loss of transformation efficiency
- Applications
- Cloning of DNA from:
- PCR products
- Plasmids
- Cosmids
- Genomic, phage and viral DNA
- Linker ligation
- Re-ligation of linearized plasmids
- Ligation of double-stranded oligonucleotides into vectors
(plasmid and phage)
- Description
Quick-Stick Ligase is designed to carry out fast and efficient ligation of both
cohesive and blunt-ended DNA at room temperature. Quick-Stick Ligase
is a T4 DNA Ligase that has been mutated to improve enzyme activity,
and contains a specially developed 4x Quick-Stick Buffer. The enzyme
catalyses the joining of two strands of DNA between the 5’-phosphate and
the 3’-hydroxyl groups of adjacent nucleotides in either a blunt-ended or
cohesive-ended configuration.
Quick-Stick Ligase will ligate 99% of l/HindIII cohesive-ended fragments,
or 80% of l/EcoRV blunt-ended fragments, in 5 minutes at room
temperature. 100% ligation of blunt-ended fragments can be achieved by
increasing the ligation time to 15 minutes at room temperature.
- Concentration
10u/μl
- Storage Conditions
Quick-Stick Ligase can be stored for 12 months at -20°C.
- Shipping Conditions
On Blue Ice
- Product Citations
1. Eloe, E.A., et al. Appl. Envir. Microbiol. 74, 6298-6305 (2008).
2. Nettleship, J.E., et al. Prot. Express. Purif. 62(1), 83-89 (2008).
3. Burns, C., et al. Fungal Gene. Biol. 42(4), 319-327 (2005).
4. Sevastsyanovich, Y.R., et al. Mol. Microbiol. 57(3), 819 (2005).
5. Hermann, B.P., et al. Mol. Endocrinol. 19(8), 2112-2131 (2005).
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