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| SensiMix™ Technology |
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| What is SensiMix? |
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| SensiMix is a series of kits that have been developed to provide the specificity, sensitivity and reproducibility required to deliver outstanding results in the real-time quantification of DNA and RNA from various sources.
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| The Components |
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- Our proprietary hot-start polymerase: heat activated to prevent non-specific amplification.
- Ultra-pure dNTPs: less impurities equals better assays.
- Buffers, additives and stabilisers: provide a primer-dimer minimising formulation.
- Internal reference (pre-mixed in some formulations): essential for normalisation in some block-based machines.
Each mix is extensively quality-controlled to ensure consistency and purity.
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| Features Of Our Mixes |
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- A comprehensive range: designed to deliver outstanding results in real-time PCR experiments on DNA and RNA templates.
- Validated with all major real-time instruments: to generate reliable and highly reproducible data on all commonly used real-time PCR instruments.
- Powered by SensiTaq: our proprietary highly optimized chemical hot-start PCR enzyme, to eliminate primer-dimer formation and non-specific amplification, essential when quantifying low copy number targets and reducing background to a minimum.
- Extremely fast protocols: formulations facilitate very rapid enzyme-extension rates, enabling fast cycling conditions to be used without compromising PCR efficiency, making it ideal for the new generation of fast-cyclers.
- Flexible: formulations have been developed for a variety of DNA and RNA templates including cDNA, poly(A) RNA, total RNA and genomic templates.
- Reliable: all reagents are rigorously quality-controlled to minimize batch-to-batch variation and tested to ensure the absence of contamination.
- Applications (include): copy number variation, microRNA analysis, microarray verification, oncology, pathogen detection, quality control and assay validation, quantitation of gene expression, single cell analysis, siRNA/RNAi experiments, SNP genotyping, viral quantitation.
“With the SensiMix we have achieved very tight reproducibility.”
Dr. Alastair Goldman,
Department of Molecular Biology and Biotechnology,
University of Sheffield,
UK
“Sensimix real-time PCR reagents performed extremely well in my experiments. They produced data with high sensitivity and reproducibility and certainly represented good value for money.”
Dr. Nicholas Jakubovics,
Oral Biology,
School of Dental Sciences,
Newcastle University,
Newcastle upon Tyne,
UK.
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SensiMix for HRM™
SensiMix HRM™ has been designed for High Resolution Melt (HRM) analysis
find out more...
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