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  • Probe

    SensiFAST™ Probe has been developed for fast qPCR and is designed for superior sensitivity and specificity with probe-detection technology, including TaqMan®, molecular beacon and Scorpions® probes. A combination of the latest advances in buffer chemistry and PCR enhancers, in conjunction with an antibody-mediated polymerase promote rapid, consistently accurate detection of DNA and RNA targets from a broad range of sample types.

    • Improved consistency between technical replicates
    • Reliable quantitation of low abundance targets
    • Reproducible, accurate results in as little as 30 minutes
    • Antibody-mediated hot-start minimizes non-specific amplification
    • Excellent efficiency for improved multiplexing

    SensiFAST Two-Step Real-Time PCR Guide

    Download the SensiFAST Two-Step Real-Time PCR Guide with detailed product descriptions and performance data to help you choose the best product for your research

    Introduction to SensiFAST

    Overview, features and benefits of the SensiFAST product family

    “I performed a comparison of Bioline's SensiFAST™ Probe against several other qPCR master mixes. The Bioline product performed the best overall in terms of efficiency, sensitivity and lower cost. I have also been very impressed with Bioline's customer service.”

    Tiffany Kosch, James Cook University, Townsville, Australia

    Probe One-Step

    The SensiFAST™ Probe One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube and uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR. This also gives SensiFAST Probe One-Step unbeatable efficiency in multiplexing. SensiFAST Probe One-Step Kits consists of a 2x SensiFAST Probe One-Step mix, separate reverse transcriptase and RiboSafe RNase Inhibitor.

    • Novel 1-step buffer optimized for highly-efficient RT and PCR steps
    • Improved consistency between technical replicates
    • Reproducible, accurate results in as little as 40 minutes
    • Antibody-mediated hot-start minimizes non-specific amplification
    • Excellent efficiency for improved multiplexing

    SensiFAST One-Step Real-Time PCR Guide

    Download the SensiFAST One-Step Real-Time PCR Guide with detailed product descriptions and performance data to help you choose the best product for your research

    Real-Time PCR Selection Chart

    Select the best reagent for your research based on your instrument and sample requirements

    “Very good linearity right down to 10 copies, very good correlation coefficient, good qPCR reaction efficiency and gave a single band on an agarose gel.”

    King’s College London, UK

    SYBR®

    The SensiFAST™ SYBR® Kit has been developed for fast, highly-sensitive and reproducible qPCR and has been validated on commonly-used real-time PCR instruments. The use of an antibody-mediated hot-start DNA polymerase minimizes amplification from primer-dimers, thereby improving assay specificity and sensitivity.

    • Improved consistency between technical replicates
    • Reliable quantitation of low abundance targets
    • Reproducible, accurate results in as little as 30 minutes
    • Antibody-mediated hot-start minimizes non-specific amplification

    SensiFAST Two-Step Real-Time PCR Guide

    Download the SensiFAST Two-Step Real-Time PCR Guide with detailed product descriptions and performance data to help you choose the best product for your research

    Introduction to SensiFAST

    Overview, features and benefits of the SensiFAST product family

    SYBR® One-Step

    SensiFAST™ SYBR® One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The SensiFAST SYBR® One-Step Kits use a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR. SensiFAST One-Step Kits consist of a 2x SensiFAST SYBR® One-Step mix, plus separate reverse transcriptase and RiboSafe RNase Inhibitor.

    • Optimized 1-step buffer for highly-efficient reverse transcription and amplification
    • Improved consistency between technical replicates
    • Reproducible, accurate results in as little as 40 minutes
    • Antibody-mediated hot-start minimizes non-specific amplification
    • Minimized primer-dimer amplification for improved specificity and sensitivity

    SensiFAST One-Step Real-Time PCR Guide

    Download the SensiFAST One-Step Real-Time PCR Guide with detailed product descriptions and performance data to help you choose the best product for your research

    Real-Time PCR Selection Chart

    Select the best reagent for your research based on your instrument and sample requirements

    “Very good linearity right down to 10 copies, very good correlation coefficient, good qPCR reaction efficiency and gave a single band on an agarose gel.”

    King’s College London, UK

    Genotyping

    The SensiFAST™ Genotyping Kit has been developed for high-performance dual-labelled probe assays that deliver fast, precise and highly reproducible characterization of sequence variants, including loci with type IV SNPs. The optimized buffer system promotes clear allelic discrimination and outstanding allele clustering.

    • Clear discrimination of all alleles
    • Tight fluorescence clusters
    • Reproducible results in as little as 30 minutes
    • Reliable genotyping from even very limiting amounts of sample
    • Genotyping from a wide range of samples


    SensiFAST™ HRM Kit is a post-PCR analysis method of characterizing nucleic acid samples based on their DNA strand dissociation behavior, with increasing temperature, in the presence of saturating fluorescent dyes. Previously unknown and even complex sequence variations can be easily detected.

    • Clear discrimination of even the most challenging sequence differences
    • Unparalleled consistency between replicate difference plots
    • Efficient detection from limiting amounts of sample
    • Reliable characterization of a broad range of sequence differences
    • Rapid PCR amplification for increased throughput

    SensiFAST Genotyping Kit

    Overview, features and benefits of the SensiFAST Genotyping Kit

    SensiFAST HRM Kit

    Overview, features and benefits of the SensiFAST HRM Kit

    “Very good linearity right down to 10 copies, very good correlation coefficient, good qPCR reaction efficiency and gave a single band on an agarose gel.”

    King’s College London, UK

    cDNA Synthesis

    For 2-step RT-qPCR, Bioline has developed the SensiFAST™ cDNA Synthesis Kit to complement the SensiFAST Probe and SYBR® Kits. The unique combination of our high-performance reverse transcriptase and optimized TransAmp™ buffer system delivers excellent reverse transcription efficiency across a very broad range of input RNA concentrations.

    • Unique reverse transcriptase and TransAmp™ buffer generate consistent first-strand cDNA
    • Reliable analysis from as little as 1 pg of starting total RNA
    • Broad dynamic range enabling quantification of dilute and low-copy samples
    • Unbiased cDNA synthesis for complete 5’ to 3’ RNA sequence representation
    • Reverse transcription and qPCR complete in less than 60 minutes

    Product

    SensiFAST cDNA Synthesis Kit

    Overview, features and benefits of the SensiFAST cDNA Synthesis Kit

    “Very good linearity right down to 10 copies, very good correlation coefficient, good qPCR reaction efficiency and gave a single band on an agarose gel.”

    King’s College London, UK

    Reviews

    "I have been using a Bioline's qPCR mix (SensiFAST SYBR No-ROX, BIO-98020) ..."

    "Very good linearity right down to 10 copies, very good correlation coeffic ..."

    "Great product, worked exactly how expected, and sensitive to small amounts ..."

    "SensiFAST SYBR No-ROX Kit is a wonderful product, it makes all the differe ..."

    "In the course of optimizing real-time PCR for analysis of various animal t ..."