SensiFAST™ Probe has been developed for fast, highly specific and reproducible real-time PCR and has been validated on all commonly used real-time instruments. The kit has been formulated for use with probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes. A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that the SensiFAST Probe Kit delivers fast, reproducible, highly-specific and ultra-sensitive real-time PCR, making SensiFAST Probe ideal for multiplexing.

  • Highly specific – minimal non-specific activity leading to better efficiency
  • Ultra-sensitive – perfect for low copy number samples
  • Fast - unique buffer chemistry allowing fast protocols and higher productivity
  • Efficient multiplexing – no loss in efficiency using multiple probes

SensiFAST Probe Hi-ROX Kit

SensiFAST Probe Lo-ROX Kit

SensiFAST Probe No-ROX Kit


SensiFAST™ SYBR® Kits are a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensuring that the SensiFAST SYBR® Kits produces fast, highly-specific, reproducible and ultra-sensitive real-time PCR. For ease-of-use and added convenience, the kits are provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilisers and enhancers.

  • Accurate quantification - hot-start capability reduces primer-dimer formation and saves time
  • Sensitive - from low copy targets
  • Rapid - unique buffer chemistry for highest specificity and sensitivity
  • Flexible - compatible with all standard and fast cycling instruments

SensiFAST SYBR® & Fluorescein Kit

SensiFAST SYBR® Hi-ROX Kit

SensiFAST SYBR® Lo-ROX Kit

SensiFAST SYBR® No-ROX Kit


SensiFAST™ SYBR® One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The SensiFAST SYBR® One-Step Kits uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR. SensiFAST One-Step Kits consist of a 2x SensiFAST SYBR® One-Step mix, plus separate reverse transcriptase and RiboSafe RNase Inhibitor.

  • Accurate quantification - hot-start capability reduces primer-dimer formation and saves time
  • Sensitive - from low copy targets
  • Rapid - unique buffer chemistry for highest specificity and sensitivity
  • Flexible - compatible with all standard and fast cycling instruments

SensiFAST SYBR® & Fluorescein One-Step Kit

SensiFAST SYBR® Hi-ROX One-Step Kit

SensiFAST SYBR® Lo-ROX One-Step Kit

SensiFAST SYBR® No-ROX One-Step Kit


SensiFAST™ Probe One-Step Kits have been developed for fast RT-qPCR and has been validated on all commonly used real-time instruments. The kit is designed for superior sensitivity and specificity with probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes. The SensiFAST Probe One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube and uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR. This also gives SensiFAST Probe One-Step unbeatable efficiency in multiplexing. SensiFAST Probe One-Step Kits consists of a 2x SensiFAST Probe One-Step mix, separate reverse transcriptase and RiboSafe RNase Inhibitor.

  • Rapid - optimized for fast reverse transcription real-time PCR
  • Accurate quantification - for calculating gene expression from RNA
  • Sensitive – unique buffer chemistry gives superior sensitivity of low copy targets
  • Flexible - compatible with all standard and fast cycling instruments

SensiFAST Probe Hi-ROX One-Step Kit

SensiFAST Probe Lo-ROX One-Step Kit

SensiFAST Probe No-ROX One-Step Kit


Single nucleotide polymorphisms (SNPs) are single-base variations at specific sites in the genome. The frequency of SNPs is about one in 300bp in the human genome, making it one of the most frequent sequence variations. High resolution melt (HRM) and duel probe-based analysis, are examples of methods used for SNP genotyping. SensiFAST HRM Kit is a post-PCR analysis method of characterizing nucleic acid samples based on DNA strand dissociation behaviour during transition from double-stranded DNA to single-stranded DNA with increasing temperature and is low throughput. SensiFAST Genotyping Kit uses a dual-labeled probe assay fast, precise and highly reproducible genotyping and can be used for high throughput.

  • Accurate allele calling – clear allele discrimination for higher confidence genotype calling
  • Tight clustering, easy analysis – perfect for difficult SNPs
  • Optimized - Assays quickly and reliably established, even with difficult genomic loci
  • Cost effective - Ideal for large scale genotyping projects

SensiFAST™ Lo-ROX Genotyping Kit

SensiFAST™ Hi-ROX Genotyping Kit

SensiFAST HRM Kit


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SensiFAST Compatibility List
SensiFAST SYBR Hi-ROX Kit
ABI 7000, 7300, 7700, 7900, 7900HT, 7900HT FAST, StepOne, StepOne Plus
SensiFAST SYBR Lo-ROX Kit
ABI 7500, 7500 FAST, ViiA7, Stratagene (Agilent) Mx4000, Mx3000P, Mx3005P
SensiFAST SYBR No-ROX Kit
Roche LightCycler 480 platform, LightCycler Nano, Bio-Rad Opticon, Opticon2, Chromo4, MiniOpticon, CFX96, CFX384, Cepheid SmartCycler, Qiagen Rotor-Gene 3000, Rotor-Gene 6000, Rotor-Gene Q, Eppendorf Mastercycler ep realplex, PCRmax Eco, Takara Thermal Cycler Dice (TP800), Analytik Jena qTOWER and Techne Quantica, PrimeQ.
SensiFAST Probe Hi-ROX Kit
ABI 7000, 7300, 7700, 7900, 7900HT, 7900HT FAST, StepOne, StepOne Plus
SensiFAST Probe Lo-ROX Kit
ABI 7500, 7500 FAST, ViiA7, Stratagene (Agilent) Mx4000, Mx3000P, Mx3005P
SensiFAST Probe No-ROX Kit
Roche LightCycler 480 platform, LightCycler Nano, Bio-Rad Opticon, Opticon2, Chromo4, MiniOpticon, CFX96, CFX384, Cepheid SmartCycler, Qiagen Rotor-Gene 3000, Rotor-Gene 6000, Rotor-Gene Q, Eppendorf Mastercycler ep realplex, PCRmax Eco, Takara Thermal Cycler Dice (TP800), Analytik Jena qTOWER and Techne Quantica, PrimeQ
SensiFAST SYBR Hi-ROX One-Step Kit
ABI 7000, 7300, 7700, 7900, 7900HT, 7900HT FAST, StepOne, StepOne Plus
SensiFAST SYBR Lo-ROX One-Step Kit
ABI 7500, 7500 FAST, ViiA7, Stratagene (Agilent) Mx4000, Mx3000P, Mx3005P
SensiFAST SYBR No-ROX One-Step Kit
Roche LightCycler 480 platform, LightCycler Nano, Bio-Rad Opticon, Opticon2, Chromo4, MiniOpticon, CFX96, CFX384, Cepheid SmartCycler, Qiagen Rotor-Gene 3000, Rotor-Gene 6000, Rotor-Gene Q, Eppendorf Mastercycler ep realplex, PCRmax Eco, Takara Thermal Cycler Dice (TP800), Analytik Jena qTOWER and Techne Quantica, PrimeQ
SensiFAST Probe Hi-ROX One-Step Kit
ABI 7000, 7300, 7700, 7900, 7900HT, 7900HT FAST, StepOne, StepOne Plus
SensiFAST Probe Lo-ROX One-Step Kit
ABI 7500, 7500 FAST, ViiA7, Stratagene (Agilent) Mx4000, Mx3000P, Mx3005P
SensiFAST Probe No-ROX One-Step Kit
Roche LightCycler 480 platform, LightCycler Nano, Bio-Rad Opticon, Opticon2, Chromo4, MiniOpticon, CFX96, CFX384, Cepheid SmartCycler, Qiagen Rotor-Gene 3000, Rotor-Gene 6000, Rotor-Gene Q, Eppendorf Mastercycler ep realplex, PCRmax Eco, Takara Thermal Cycler Dice (TP800), Analytik Jena qTOWER and Techne Quantica, PrimeQ

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  • SensiFAST Instrument Compatibility List

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  • Product: SensiFAST™ Probe No-ROX Kit

    Review: I performed a comparison of of Bioline's SensiFAST™ Probe No-ROX Kit ...

      I performed a comparison of of Bioline's SensiFAST™ Probe No-ROX Kit (cat. BIO-86005) against several other qPCR master mixes. The Bioline product performed the best overall (i.e. better efficiency and sensitivity, lower cost). I have attached a graph of the Quantitation analysis from my trial run of this product. I have also been very impressed Bioline's customer service and really like that they are willing to send free samples of most of their products.
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    Tiffany Kosch, James Cook University, Townsville, Australia

  • Product: SensiFAST™ cDNA Synthesis Kit

    Review: I have been using Bioline products for past 5 years ...

      I have been using Bioline products for past 5 years, starting with MyTaqRed Mix and dNTPs. Recently, we started using the SensiFAST cDNA Synthesis Kit and SensiFAST SYBR mix for qRT-PCR. Upon testing with diverse RNA sources, we were really satisfied with the results as the data is quite comparable with competitor SYBR mixes. Quality to price ratio is the winning factor for both the cDNA synthesis kit and SYBR mix. We felt real ease-of-use with the cDNA synthesis kit, which was fast and simple. I recommend the SensiFAST cDNA Synthesis kit and SensiFAST SYBR mix from Bioline.
  • View Results:

      No images attached.
    Lakshmi Palam, Indiana University School of Medicine, Indianapolis, US

  • Product: SensiFAST™ Probe Hi-ROX Kit

    Review: Our lab has been routinely been using the Bioline SensiFAST Probe ...

      Our lab has been routinely been using the Bioline SensiFAST Probe Hi-ROX for microRNAs detection in mammalian cells for the past 3 years. Results are highly reproducible between technical replicates and independent experiments. We found that this product showed high sensitivity, low background noise and consistent amplification curves. It performs as well if not better than competitor products. In addition, several thawing/freezing cycles do not influence its activity.
      Given its low cost, SensiFAST Probe Hi-ROX is a really attractive option compared to competitor products. Overall, we are completely satisfied and would recommend it to other labs.
  • View Results:

      No images attached.
    Charlotte Nejad, Hudson Institute of Medical Research, Clayton, Australia

  • Product: SensiFAST™ cDNA Synthesis Kit

    Review: Working with post-mortem human heart tissue samples for a gene ...

      Working with post-mortem human heart tissue samples for a gene expression project proved to be a challenging task for tissue samples that came from the medical examiner's office. So many factors were working against a viable gene expression profile for these samples such as time of death to autopsy lag, time from autopsy to tissue sample, and whether or not the tissue had been flash frozen. The samples were also never treated with RNA-later so we expected highly degraded RNA from these tissue samples.

      Our only light of hope was the fact that our target amplicon sizes were less than 100 bp which meant that we could still find what we were looking for in spite of highly degraded RNA. Chances were that at least a few intact sequences would amplify. The critical part in our experiment was to be able to yield enough cDNA from whatever bit of RNA we were able to extract from our samples.

      This is where Bioline exceeded all expectations. Working quickly and carefully, I was able to get decent cDNA considering all the factors working against my RNA isolations. The cDNA was enough to be able to carry out a gene expression profile for each of the samples and our project was a success. Bioline proved to be superior in cDNA yield from highly degraded RNA samples.
  • View Results:

    Daniel Perez, Heart Institute, Los Angeles, US

  • Product: SensiFAST™ SYBR No-ROX Kit

    Review: We use the SensiFAST SYBR No-ROX Kit and the Rotor-Gene ...

      We use the SensiFAST SYBR No-ROX Kit and the Rotor-Gene 6000 for gene expression studies on fruit crops. We tested different kits and the Bioline kit has excellent sensitivity and a competitive price level.
  • View Results:

    Patrick Winterhagen, University of Hohenheim, Stuttgart, Germany

  • Product: SensiFAST™ SYBR No-ROX Kit

    Review: We use the SensiFAST SYBR No-ROX Kit and the Rotor-Gene ...

      We use the SensiFAST SYBR No-ROX Kit and the Rotor-Gene 6000 for gene expression studies on fruit crops. We tested different kits and the Bioline kit has excellent sensitivity and a competitive price level.
  • View Results:

    Patrick Winterhagen, University of Hohenheim, Stuttgart, Germany

  • Product: SensiFAST™ SYBR® Hi-ROX Kit

    Review: I have tested SensiFAST SYBR Hi-ROX using mouse ...

      I have tested SensiFAST SYBR Hi-ROX using mouse cDNA samples, on a StepOnePlus machine, in fast and regular mode, and in comparison with our current SYBR Green mix. The test was globally positive, in favor of the SensiFAST product.
  • View Results:

    Alice Jouneau, INRA, Jouy-en-Josas, France

  • Product: SensiFAST™ SYBR No-ROX Kit

    Review: Amazing! Just take a look to the comparison I have ...

      Amazing! Just take a look to the comparison I have made! And I did some others as well: thaw/freeze cycles (4 times without any change), primers specificities (no changes). All the tests were free using small aliquots from Bioline. Only one thing would still stop you? The price is the lowest I've ever had. To me, using my conditions and all the tests I ran, this is the best mix for qPCR!
  • View Results:

    Florent Hube, CNRS, Paris, France

  • Product: SensiFAST™ SYBR No-ROX Kit

    Review: Excellent amplification of Oct4 in induced ...

      Excellent amplification of Oct4 in induced pluripotent stem cells from 74ng/rx to 0.9ng/rx on Roche Lightcycler 480 with an efficiency of 1.98.
  • View Results:

    Martin Engel, University of Wollongong, Wollongong, Australia

  • Product: SensiFAST™ SYBR No-ROX Kit

    Review: We tested the SensiFAST™ SYBR® No-ROX Kit against the ...

      We tested the SensiFAST™ SYBR® No-ROX Kit against the LightCycler® 480 SYBR Green I Master on a Roche Lightcycler 480. The cycling conditions we used were optimized for the Roche kit. Despite this, the SensiFAST kit performed significantly better, leasing to earlier amplification from comparable genomic DNA samples (see picture for example). This gives us the increased sensitivity we were seeking. On top of that it is much cheaper than the Roche mix. I highly recommend this product.
  • View Results:

    Andrew Taylor, University of Warwick, Warwick, UK

  • Product: SensiFAST™ SYBR Lo-ROX Kit

    Review: I had a very good experience with ...

      I had a very good experience with the SensiFAST SYBR Lo-ROX qPCR master mix. It was quick and easy to use, while also providing me with near perfect R-squared values and dissociation curves. Even when compared to other, more expensive, master mixes, I obtained results of equal or better quality.
  • View Results:

      No images attached.
    Mariah Meyer, The University of Montana, DBS, US

  • Product: SensiFAST™ Probe No-ROX One-Step Kit

    Review: "I compared the One-Step RT PCR master mix of Bioline ..."

      "I compared the One-Step RT PCR master mix of Bioline (SensiFAST Probe No-ROX One-Step Kit) with a commercial leading product (called QF here). The higher sensitivity of SensiFAST Probe No-ROX can be reflected by producing lower CT value. Also, this product is in a good price range among the other commercial product. Value for money!!"
  • View Results:

    Peony Fung, Cerberus Sciences, Thebarton, Australia

  • Product: SensiFAST™ SYBR No-ROX Kit

    Review: "We are using the Bioline SensiFAST SYBR No-Rox Mix for..."

      "We are using the Bioline SensiFAST SYBR No-Rox Mix for our qPCR studies. We have compared the mix to the Roche Lighcycler 480 SYBR Green I Master Mix. With the Bioline SensiFAST mix, our targets regularly appear at lower Cp values and all primers tested work more reliably and consistently (see attached table for two examples of primer efficiencies determined with the Lightcycler and the SensiFast kits). Furthermore, Bioline technical support always answered our queries very promptly and in great detail and the SensiFAST mix costs us less than half the price of the Lightcycler Mix."
  • View Results:

    Anne Schlaitz, Heidelberg University, Heidelberg, Germany

  • Product: SensiFAST™ SYBR Lo-ROX Kit

    Review: "I really like the SensiFAST SYBR Green (Lo-Rox) kit because..."

      "I really like the SensiFAST SYBR Green (Lo-Rox) kit because it always removes the background noise and gives clean curves."
  • View Results:

      No images attached.
    Xiaowei Xu, Baylor college of medicine, Houston, USA

  • Product: SensiFAST Probe Hi-ROX

    Review: We compared the Bioline SensiFAST Probe Hi-ROX ...

      We compared the Bioline SensiFAST Probe Hi-ROX master mix to a qRT-PCR master mix supplied by a leading competitor for our microRNA qRT-PCR studies. We found the Bioline product to consistently produce lower Ct values, as well as being more cost-effective.
  • View Results:

    Rachel Crossland, Newcastle University, Newcastle upon Tyne, UK

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