| Sign In | Quick Order CART (0)
  • Ordering

    Cat. No.
    Size
    List Price*
    QTY
    BIO-33045
    200 Lanes
    £57.50
    +
    BIO-33046
    500 Lanes
    £124.50
    +
    *To check your pricing please Login or contact us

    Description

    Ready-to-use molecular weight marker, designed for fast size determination of nucleic acids from 100 bp to 2000 bp.

    Product Highlights

    • Broad size range - 5 evenly distributed bands from 50 bp to 2000 bp
    • Ready-to-use - reduces handling steps and saves time
    • Fast separation - ideal for PCR products
    • Supplied with loading dye - for loading sample DNA

    Product Description

    Agarose gels are often run to check and see if a band is present or not, for example in a PCR reaction.  With 5 regularly spaced bands, ranging from 100 bp to 2000 bp, EasyLadder™ I is a molecular weight marker especially designed for fast size determination of linear double-stranded DNA fragments on 0.5% to 3% TAE or TBE agarose gels.

    This ready-to-use format, with a red loading dye already added to the ladder, reduces handling steps and saves time; simply transfer EasyLadder I from the vial to the gel, along with the PCR product and run at high voltage for just a few minutes (1-3 cm of a gel lane). The concentration of DNA in each of the EasyLadder I bands is provided for optionally determining the approximate mass of DNA in comparably intense sample bands of similar size. The EasyLadder I is also supplied with a 5x loading dye for the loading of sample DNA.

    Applications

    • Determination of PCR amplicon size
    • Confirmation of plasmid construction
    • Restriction fragment size determination
    Main
    Ready-to-use molecular weight marker for fast size determination.

    Specification

    Components

    Reagent

    100 Lanes

    200 Lanes

    500 Lanes

    EasyLadder I

    500 µL

    2 x 500 µL

    5 x 500 µL

    5x Sample Loading Buffer

    1 mL

    1 mL

    1 mL

    Storage & Stability

    All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.

    Components may also be stored at +4°C or room temperature if required, although storage at -20°C is always recommended.

    When stored at +4°C, EasyLadder I can be stored for up to 12 months. When stored at room temperature, EasyLadder I can be stored for up to 1 month

    Shipping conditions

    Ambient temperature.



    FAQs

    All HyperLadders and EasyLadders are ready-to-load and require no addition of loading buffer prior to use. In general, the smaller the DNA product, the higher the percentage of Agarose required in the gel. As a guide for optimization we would suggest to use the EasyLadder I with 2% (w/v) agarose gel,

    Hyperladder 100bp with 2% (w/v) agarose gel,

    Hyperladder 1kb with 1% (w/v) agarose gel,

    Hyperladder 50bp with 1.5% (w/v) agarose gel and

    Hyperladder 25bp with 3.5% (w/v) agarose gel.

    Please have a look into the related application note for more information about ladder migration in different gel concentrations.



    Problems with our HyperLadder and EasyLadder are extremely rare, so please make sure you have carried out each of the following correctly:

    -          Used the correct percentage of agarose in your gel for the specific marker in question.

    -          Used 5 µL of the marker per lane.

    -          Used the correct concentration of stain (e.g. EtBr) to visualise correctly.

    -          Vortexed the marker gently before use.

    Should problems persist, please contact technical services for further assistance.



    No pre-treatment, such as denaturing, is necessary prior to use.

    No, our Ladder range is supplied in a ready-to-load format to be directly loaded onto your agarose gel. An additional vial of 5x Loading Buffer is supplied with each of our markers for use with your samples.



    Our HyperLadder and EasyLadder range is designed for use on Agarose gels and we do not recommend them for PAGE. However, should you wish to use Bioline HyperLadders in this technique, HyperLadder 25bp, our small fragment DNA marker, would be the most appropriate.

    Other stains, such as SYBR® Green Gel Stain, can be used with our ladders as effectively as EtBr. The fluorescence of the gel as a whole may vary depending on the stain used, however the relative intensities of the marker bands and of the samples run will remain the same, thus the HyperLadder / EasyLadder remains quantitative.



    EasyLadder I: gel with 2% (w/v) agarose in TBE

    HyperLadder 1kb: gel with 1% (w/v) agarose in TBE

    HyperLadder 100bp: gel with 2% (w/v) agarose in TBE

    HyperLadder 50bp: gel with 1,5% (w/v) agarose in TBE

    HyperLadder 25bp: gel with 3,5% (w/v) agarose in TBE

    For all 5 µL ladder have been applied and visualized using ethidium bromide.

     



    The EasyLadder and the related red DNA loading buffer contain cresol red. The HyperLadder and the related blue DNA loading buffer contain bromophenol blue.