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    Cat. No.
    Size
    List Price*
    QTY
    BIO-25054
    250 Reactions
    £196.00
    +
    *To check your pricing please Login or contact us

    Description

    MyTaq™ Blood-PCR Kit offers very fast, highly-specific, direct PCR from a wide range of human and animal whole blood samples, including those preserved with anticoagulants.

    Product Highlights

    • Fast – eliminate complex, slow and costly DNA extraction steps, thereby reducing time to results
    • Sensitive – incorporates MyTaq HS DNA Polymerase that exhibits increased affinity for DNA, thereby improving yield of even the most challenging targets
    • Robust – novel buffer system developed to overcome blood inhibition for highest PCR success rates and improved sensitivity
    • Flexible – developed for a wide range of blood samples, including EDTA, citrate and heparin preserved samples, thereby avoiding the need for further optimization
    • Versatile – developed for a range of PCR applications, including multiplexing, amplification of GC rich templates and long amplicons

    Product Description

    MyTaq™ Blood-PCR Kit is recommended for fast, specific and direct PCR from human and animal blood samples. MyTaq Blood-PCR Kit incorporates MyTaq HS DNA Polymerase, a next-generation hot-start polymerase that delivers highly-specific PCR amplification. Furthermore, MyTaq HS has an increased affinity for template DNA, giving a high PCR product yield for most challenging templates. MyTaq HS DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases, meaning it performs reliably even in the presence of PCR inhibitors.

    The combination of a unique, inhibitor-tolerant buffer system and MyTaq HS, ensures the MyTaq Blood-PCR Kit overcomes the PCR inhibitors typically present in blood samples, including anticoagulants (EDTA, citrate and heparin). This leads to significantly increased sensitivity and PCR success rates even with demanding applications such as GC-rich templates or longer amplicons.

    The speed and high specificity of MyTaq Blood-PCR Kit also makes it highly suited for multiplex PCR and high-throughput genotyping assays. The advanced formulation of MyTaq Blood-PCR Kit allows fast cycling conditions to be used, without compromising PCR specificity and yield. In addition to supporting robust PCR amplification, the novel buffer system replaces the need for complicated extraction or purification steps or use of additives.

    Applications

    • SNP genotyping
    • Genetic testing
    • Pathogen detection
    • Blood screening
    • Paternity testing
    Main
    Highly efficient performance in singleplex and multiplex assays.

    MyTaq Blood-PCR Kit Customer Review

    PCR Selection Chart

    Select the best reagent for your research

    PCR Enzyme Guide

    Download the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your research

    Application Note

    MyTaq™ Blood-PCR Kit

    I’ve tested a number of products and by far the best one was MyTaq Blood-PCR Kit. The major advantage is that it works on blood samples and crude lysates. This product will also amplify larger amplicons than other products tested, even in a multiplex scenario. Other key advantages include the hot-start and its ability to withstand 30 freeze-thaw cycles.

    Markus Zeller, AutoGenomics, California, US

    Product Selection

    Please refer to the PCR Selection Chart to confirm the recommended product for your PCR application.


    Specification

    Components

    Reagent

    100 Reactions

    250 Reactions

    MyTaq Blood-PCR Mix, 2x

    2 x 625 µL

    5 x 625 µL

    Concentration

    2x

    Storage & Stability

    MyTaq Blood-PCR Kit can be stored at -20°C. Repeated freeze/thaw cycles should be avoided.

    When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date printed on the outer box label.

    Shipping conditions

    On dry or blue ice.



    FAQs

    All our polymerases are guaranteed for a period of 12 months from the date of purchase. These should be stored at -20°C during this time for optimal retention of activity.

    Please Note: We do not recommend the storage of our polymerases at -80 °C as ice crystals could form on the active site, which may affect or destroy the activity of the enzyme.

    PCR can be a challenging technique, with various parameters to optimize to achieve the best results. If you are having problems, these could be easily resolved by addressing a few issues. Please see our PCR troubleshooting guide for suggestions and help with your specific problems.

    Observation Recommended Solution(s)
    No or low PCR yield  Enzyme concentration too low – increase the amount of enzyme in 0.5 U increments.
     Primers degraded – check quality and age of the primers.
     Magnesium concentration too low – increase concentration in 0.25 mM increments with a starting  concentration of 1.75 mM.
     Primer concentration not optimized. Titrate primer concentration (0.3-1 µM); ensuring that both  primers have the same concentration.
     Template concentration too low – Increase concentration of template.
     Perform a positive control to ensure that the enzyme, dNTPs and buffers are not degraded and/or contaminated.
    Multiple Bands  Primer annealing temperature too low. Increase annealing temperature. Primer annealing should be at least 5°C below the calculated Tm of primers.
     Prepare master mixes on ice or use a heat-activated polymerase.
     For problems with low specificity. Try adding 3% DMSO (not supplied) to improve specificity.
    Smearing or artifacts  Template concentration too high. Prepare serial dilutions of template.
     Too many cycles. Reduce the cycle number by 3-5 to remove non-specific bands.
     Enzyme concentration too high - decrease the amount of enzyme in 0.5 U increments.
     Extension time too long. Reduce extension time in 0.5-1 minute increments.


    During PCR setup at room temperature, standard polymerases will have some activity. When using a hot-start polymerase, this enzyme will have no activity at room temperature, thus reducing the risk of unspecific products in the reaction due to these mis-primed oligonucleotides. This feature makes this type of polymerase ideally suited to high-throughput applications, where the reactions may be left sitting at room temperature for prolonged periods of time.

    Please click here in order to request your sample. You will receive an email confirmation within two business days with delivery details.