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    Cat. No.
    Size
    List Price*
    QTY
    BIO-65053
    50 Reactions
    £212.00
    +
    BIO-65054
    250 Reactions
    £971.00
    +
    *To check your pricing please Login or contact us

    Description

    Highly-efficient, full-length reverse transcription of even the most challenging RNA templates, under fast conditions.

    Product Highlights

    • Efficient – high target affinity, coupled with a novel TransAmp™ buffer system for improved yield of full length cDNA
    • Unbiased – optimized mix of random hexamers and anchored oligo dT primers for complete 5’ to 3’ RNA sequence representation
    • Sensitive – lower Ct values from a broad range of input cDNA concentrations enabling accurate detection of dilute (1 pg of input RNA) and even very low copy targets
    • Robust – reliable reverse transcription under challenging conditions, including complex templates and in the presence of inhibitors
    • Fast – high-yield reverse transcription from a very broad of targets in as little as 5 minutes

    Product Description

    To complement the SensiFAST™ Probe and SYBR® kits, Bioline has developed the SensiFAST™ cDNA Synthesis Kit to provide a rapid and sensitive method for first-strand cDNA synthesis. The SensiFAST™ cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in cDNA templates, regardless of gene abundance, making it excellent for use in real-time PCR studies.

    A novel, highly-pure reverse transcriptase and new TransAmp™ buffer system delivers highly-efficient synthesis of cDNA, enhanced reproducibility and data accuracy. These features make the SensiFAST™ cDNA Synthesis Kit ideal for working with limited samples, such as laser-micro dissected samples and tissue biopsies. The TransAmp Buffer also employs a unique blend of random hexamer primers and anchored oligo dT to ensure unbiased 3’ and 5’ coverage and reverse transcription of all regions. Additionally, the SensiFAST™ cDNA Synthesis Kit can be used with SensiFAST™ Probe and SYBR® Kits for fast real-time RT-PCR without compromising quality, with real-time results in less than an hour.

    Applications

    • Gene expression analysis
    • Tissue biopsy analysis
    • miRNA profiling / quantification
    • RNA target detection
    • Pathogen detection
    Main
    Outstanding sensitivity under fast conditions.

    SensiFAST cDNA Synthesis Kit Customer Review

    SensiFAST cDNA Synthesis Kit Customer Review

    One-step Vs. Two-step real-time RT PCR

    A discussion of the pros and cons of each detection strategy.

    Our Institute consisting of more than 60 active researchers has a longstanding interest in understanding changes to the transcriptional landscape of prostate cancer. Reproducibility, sensitivity and cost-effectiveness are critical parameters in selecting our day-to-day cDNA synthesis kit. This combined with its convenient two reagent system continues to make the SensiFast cDNA synthesis kit stand out from its competitors and our preference for almost two years.

    Martin Sadowski, Queensland University of Technology, Brisbane, Australia

    Product Selection

    Please refer to the Real-Time PCR Selection Chart to confirm the recommended qPCR mix for your real-time PCR application and instrument.


    Resources

    Certification of Analysis (COAs)

    Specification

    Components

     Product Name

     50 reactions

     250 reactions

     5x TransAmp Buffer

     200 µL

     1 mL

     Reverse Transcriptase

     50 µL

     250 µL

    Storage & Stability

    SensiFAST cDNA Synthesis Kit can be stored for up to 12 months at -20°C. Repeated freeze/thaw cycles should be avoided.

    When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date printed on the outer box label.

    Shipping conditions

    On dry or blue ice.



    FAQs

    The SensiFAST cDNA Synthesis Kit has been designed for rapid generation and unbiased coverage of cDNA, especially for subsequent quantification with real-time PCR. The optimized 5x TransAmp buffer includes all necessary components for cDNA synthesis, including a unique blend of oligo dT and random hexamer primers and the RiboSafe RNase Inhibitor. The Tetro cDNA Synthesis Kit is the more flexible solution. You selects the desired setup, especially the desired RT primer. The Tetro Kit is optimized to work over a wide range of RNA concentrations and to obtain full length cDNA, which can be used for two-step PCR assays as well. Tetro is the recommended Kit for the generation of cDNA libraries and cloning.

    For SensiFAST cDNA Synthesis Kit: The TransAmp Buffer already contains reverse transcriptase enhancers that reduce complex RNA secondary structure. For templates that have a high degree of structure, such as viral RNA and some plant RNA, we suggest using an additional 15 minute 48°C incubation step as mentioned in the manual. For Tetro cDNA Synthesis Kit: For samples with high secondary structure we suggest to denature the RNA template with primers prior to the reaction set-up. Furthermore, it is necessary to optimize the incubation time and temperature. The temperature of the RT step may be raised up to a maximum of 48°C.

    The necessary amount of cDNA template for PCR or qPCR reactions may vary from the range of pg up to several hundred ng. It depends among other things on the analyzed target, the used application and sample characteristics. If you do not have empirical values for your assays please use the recommended amounts in the manuals of your PCR or qPCR assays and optimize if necessary.

    It is not possible for the SensiFAST cDNA Synthesis Kit. Random Hexamer and oligo dT primer are already included in the optimized TransAmp buffer. It is possible to use gene specific primer for the Tetro cDNA Synthesis Kit to generate cDNA for specific target.

    We recommend to use our EPIK miRNA assays to generate and analyze cDNA from miRNA. One advantage of the EPIK assays are the 3 miRNA specific primer used for cDNA synthesis and detection. They improve the specificity and enhance the discrimination of even closely related targets.

    Reviews

    "After multiple years using products from other companies, we have now trialed an ..."

    "Our Institute consisting of more than 60 active researchers has a longstanding i ..."

    "We used the Isolate II RNA Micro Kit, SensiFAST cDNA synthesis kit and SensiFAST ..."

    "We used the Isolate II RNA Micro Kit, SensiFAST cDNA synthesis kit and SensiFAST ..."

    "The SensiFast cDNA Synthesis Kit was directly compared to another cDNA synthesis ..."

    "I have been using Bioline products for past 5 years, starting with MyTaqRed Mix ..."

    "Working with post-mortem human heart tissue samples for a gene expression projec ..."

    "I had been repeating an experiment over and over again because I wasnt getting a ..."

    ""My aim is to isolate as much RNA as possible from a small amount of primar ..."