- Efficient – high target affinity, coupled with a novel TransAmp™ buffer system for improved yield of full length cDNA
- Unbiased – optimized mix of random hexamers and anchored oligo dT primers for complete 5’ to 3’ RNA sequence representation
- Sensitive – lower Ct values from a broad range of input cDNA concentrations enabling accurate detection of dilute (1 pg of input RNA) and even very low copy targets
- Robust – reliable reverse transcription under challenging conditions, including complex templates and in the presence of inhibitors
- Fast – high-yield reverse transcription from a very broad of targets in as little as 5 minutes
To complement the SensiFAST™ Probe and SYBR® kits, Bioline has developed the SensiFAST™ cDNA Synthesis Kit to provide a rapid and sensitive method for first-strand cDNA synthesis. The SensiFAST™ cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in cDNA templates, regardless of gene abundance, making it excellent for use in real-time PCR studies.
A novel, highly-pure reverse transcriptase and new TransAmp™ buffer system delivers highly-efficient synthesis of cDNA, enhanced reproducibility and data accuracy. These features make the SensiFAST™ cDNA Synthesis Kit ideal for working with limited samples, such as laser-micro dissected samples and tissue biopsies. The TransAmp Buffer also employs a unique blend of random hexamer primers and anchored oligo dT to ensure unbiased 3’ and 5’ coverage and reverse transcription of all regions. Additionally, the SensiFAST™ cDNA Synthesis Kit can be used with SensiFAST™ Probe and SYBR® Kits for fast real-time RT-PCR without compromising quality, with real-time results in less than an hour.
- Gene expression analysis
- Tissue biopsy analysis
- miRNA profiling / quantification
- RNA target detection
- Pathogen detection
Introduction to SensiFASTOverview, features and benefits of the SensiFAST product family
SensiFAST Application NoteEmploying Cell-free DNA from Maternal Plasma for Exclusion of X-linked Disorders
One-step Vs. Two-step real-time RT PCRA discussion of the pros and cons of each detection strategy.
Our Institute consisting of more than 60 active researchers has a longstanding interest in understanding changes to the transcriptional landscape of prostate cancer. Reproducibility, sensitivity and cost-effectiveness are critical parameters in selecting our day-to-day cDNA synthesis kit. This combined with its convenient two reagent system continues to make the SensiFast cDNA synthesis kit stand out from its competitors and our preference for almost two years.
Martin Sadowski, Queensland University of Technology, Brisbane, Australia
Product SelectionPlease refer to the Real-Time PCR Selection Chart to confirm the recommended qPCR mix for your real-time PCR application and instrument.
Certification of Analysis (COAs)
5x TransAmp Buffer
Storage & Stability
SensiFAST cDNA Synthesis Kit can be stored for up to 12 months at -20°C. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date printed on the outer box label.
On dry or blue ice.