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    Cat. No.
    Size
    List Price*
    QTY
    BIO-37047
    5ml
    £83.50
    +
    BIO-37048
    25ml
    £349.00
    +
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    Description

    A novel, inexpensive, column-free approach to nucleic acid recovery or concentration, ideal for use in cloning, PCR or other routine molecular biology applications.

    Product Highlights

    • Fast – simple protocol for reliable recovery of DNA and dsRNA in as little as 30 minutes
    • Efficient – column-free approach to clean-up promotes recovery of up to 98% of PCR products
    • High-performance – extraction of nucleic acid suitable for use in all routine downstream applications
    • Convenient – easy-to-use optional pink dye for improved pellet visibility, thereby minimizing sample loss

    Product Description

    SureClean Plus provides a simple, efficient method for the purification and concentration of DNA or dsRNA from PCR reactions or any enzymatic digests, without the need for hazardous reagents.  SureClean Plus removes primers, primer-dimers, dNTPs and proteins.

    The protocol allows the precipitation of nucleic acids ≥75 bp with up to 98% recovery of the original sample, without the need for organic solvents, glass milk or expensive spin columns. Samples can be resuspended in any buffer and volume of choice, thus permitting the purification process to be tailored specifically to suit downstream experimental needs.

    SureClean Plus also includes a pink co-precipitant that can be added to the sample to facilitate easy visualization of the purified pellet. Co-precipitant Pink is a synthetic polyacrylamide, designed to aid recovery of nucleic acids. The co-precipitant does not interfere with downstream applications.

    SureClean Plus has been designed to deliver optimal performance during cloning and after end point PCR.

    Applications

    • Cloning
    • End-point PCR and RT-PCR
    • qPCR and RT-qPCR
    • Southern Blotting
    • Sequencing
    • Transfection
    • Buffer exchange
    Main
    Fast, inexpensive purification or concentration of DNA or dsRNA from PCR reactions and enzymatic digests.

    Application Note

    SureClean Plus

    Specification

    Components

    Reagent

    5 mL

    25 mL

    SureClean

    1 x  5 mL

    2 x 12.5 mL

    Co-Precipitant Pink

    1 x 0.8 mL

    1 x 4 mL

    Storage & Stability

    All components should be stored at room temperature. Do not freeze. Avoid exposure of Co-Precipitant Pink to light.

    When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.



    FAQs

    SureClean Plus allows the precipitation of nucleic acids ≥75 bp, means it is possible to remove primers, primer-dimers and dNTPs as well as proteins (such as restriction enzymes, polymerases, etc.) from your sample.

    SureClean Plus also contains the Co-Precipitant Pink that can be added to the sample to facilitate easy visualization of the purified pellet. Although the Co-Precipitant Pink does not interfere with downstream applications such as cloning, PCR, real time PCR or enzymatic reactions, it is not compatible with spectrophotometric analysis of the DNA content.