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    Cat. No.
    Size
    List Price*
    QTY
    BIO-37112
    4 x 1ml
    $57.50
    +
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    Description

    5x MyTaq™ Reaction Buffer Red is an advanced formulation buffer for use with MyTaq DNA Polymerase.

    Product Highlights

    • Increased sensitivity and speed - combined with MyTaq and MyTaq HS yield and speed are significantly improved
    • All in one - includes preoptimized concentrations of dNTPs, MgCl2 stabilizers and enhancers
    • Robust - gives high yield across a wide range of templates
    • Direct gel loading - for high throughput

    Product Description

    5x MyTaq™ Reaction Buffer Red is an advanced formulation buffer that saves time and delivers superior results. The buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations which eliminate the need for further optimization.

    The specially designed red formulation does not interfere with the PCR reaction and enables users to load samples directly onto a gel after the PCR without the need to add loading buffer.

    Applications

    • Specific amplification of complex templates
    • Robust amplification of GC-rich sequences
    • High throughput PCR
    • Colony PCR
    • Multiplexing
    • Genotyping
    • Two-Step RT-PCR
    • TA cloning

    PCR Enzyme Guide

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    Specification

    Components

    Reagent

    BIO-37112

    5x MyTaq Reaction Buffer Red

    4 x 1ml

    Storage & Stability

    All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

    When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

    Shipping conditions

    Shipped on dry/blue ice



    FAQs

    No, the dyes and composition of the Red Reaction Buffers and Mixes are such that the samples will sink easily into the well and the samples can be clearly seen, thus no loading buffer is required to load your samples on an agarose gel.

    The dye present in the Red Mixes or Red Reaction Buffers does not interfere with PCR product isolation procedures or subsequent applications like sequencing. An exception is the quantification of PCR products in colored buffers with photometric methods or fluorescence assays. We cannot exclude the impact of the dye on quantification results and suggest the purification of these samples, for instance with ISOLATE II PCR & Gel kit or SureClean Plus.