Alpha-Select Bronze Efficiency

Cat No. Size List Price* Qty  
BIO-85025 2ml (10 x 200µl) $78.00
 
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α-Select Bronze Competent Cells are ideal for routine subcloning.

Features & Benefits

  • Reduced recombination of cloned DNA - designed to accommodates larger plasmids
  • High efficiency - available in ≥107 cfu/μg, perfect for routine subcloning
  • Easy screening - with blue/white color selection
  • Convenient size - small aliquots reduce waste

Applications

  • Transformation of large plasmids into bacterial cells
  • Generating clones
  • Blue/white color screening

Description

α-Select Bronze Competent Cells are directly comparable to DH5α cells. They contain a lacZ marker that provides α-complementation of the ß-galactosidase gene for blue/white color screening and are ideal for subcloning using plasmid-derived vectors. α-Select Bronze Competent Cells also provide recA1 and endA1 markers to minimize recombination and enhance the quality of the plasmid DNA, allowing efficient transformation even with large plasmids.

pUC19 DNA is also provided as a positive control.


Notes

Genotype: F- deoR endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) supE44 thi-1 phoA Δ(lacZYA-argF)U169 Φ80lacZΔM15 λ-


Components

Reagent

BIO-85025

Chemically Competent Cells

10 x 200µl

pUC19 control vector (100pg/µl)

10µl


Storage & Stability

All kit components should be stored at -80°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.


Shipping conditions

Shipped on Dry Ice.


Certificates of Analysis (COAs)

Certificates of Analysis for Alpha-Select Bronze Efficiency are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 5 COAs for Alpha-Select Bronze Efficiency →





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