BioMix™ Red is a complete ready-to-use 2x reaction mix containing an ultra-stable Taq DNA polymerase. It contains an additional inert red dye that permits easy visualization and direct loading onto a gel.
- Convenient format - pre-mixed, pre-optimized 2x solutions
- Versatile - suitable for routine PCR applications
- Ready-to-use - reduces contamination risks
- Suitable for TA cloning - leaves 'A' overhang
- Direct gel loading - eliminates the need for further processing following reaction completion
BioMix™ Red is a complete ready-to-use 2x reaction mix containing a stable Taq DNA polymerase. It contains an additional inert red dye that permits easy visualization and direct loading onto a gel. There is no need to add loading buffer as the mix is of sufficiently high density to sink to the bottom of the gel. The red dye migrates like a 350 bp fragment on a 2% agarose TAE gel (or 600 bp on a 1% agarose).
BioMix Red has been developed to perform PCR assays of many common genomic and cDNA templates; the user has simply to add water, template and primers. It reduces the time required to set-up reactions, thereby minimizing the risk of contamination. Reproducibility is ensured by reducing the number of pipetting steps that can lead to errors.
BioMix Red is supplied with additional MgCl2 solution should any fine adjustments be required.
- Routine PCR applications
- TA cloning
- High throughput
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Certification of Analysis (COAs)
2 x 1.25 mL
10 x 1.25 mL
50 mM MgCl2 Solution
- BIO-25005: 100 x 50 µL Reactions: 2 x 1.25 mL
- BIO-25006: 500 x 50 µL Reactions: 10 x 1.25 mL
Storage & Stability
All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.
Alternatively, BioMix Red can be stored for up to up to 2 weeks at +4°C.
On Dry Ice or Blue Ice.
Bioline's mixes contain magnesium chloride at the following concentrations (please see table below). An additional tube of 50 mM MgCl2 solution is supplied with each mix to facilitate further optimization if required.
|Bioline Mix||Final Magnesium Concentration|
|ACCUZYME Mix||2.0 mM.|
|BioMix / BioMix Red||2.5 mM.|
|ImmoMix / ImmoMix Red||3.0 mM.|
|BIO-X-ACT Short Mix||2.0 mM.|
Please Note: We do not recommend the storage of our polymerases at -80 °C as ice crystals could form on the active site, which may affect or destroy the activity of the enzyme.
|No or low PCR yield||Enzyme concentration too low – increase the amount of enzyme in 0.5 U increments.|
|Primers degraded – check quality and age of the primers.|
|Magnesium concentration too low – increase concentration in 0.25 mM increments with a starting concentration of 1.75 mM.|
|Primer concentration not optimized. Titrate primer concentration (0.3-1 µM); ensuring that both primers have the same concentration.|
|Template concentration too low – Increase concentration of template.|
|Perform a positive control to ensure that the enzyme, dNTPs and buffers are not degraded and/or contaminated.|
|Multiple Bands||Primer annealing temperature too low. Increase annealing temperature. Primer annealing should be at least 5°C below the calculated Tm of primers.|
|Prepare master mixes on ice or use a heat-activated polymerase.|
|For problems with low specificity. Try adding 3% DMSO (not supplied) to improve specificity.|
|Smearing or artifacts||Template concentration too high. Prepare serial dilutions of template.|
|Too many cycles. Reduce the cycle number by 3-5 to remove non-specific bands.|
|Enzyme concentration too high - decrease the amount of enzyme in 0.5 U increments.|
|Extension time too long. Reduce extension time in 0.5-1 minute increments.|