Control Mix 670 is used with the DNA Extraction Control 670, enabling users of diagnostic assays to validate their extraction step. DNA Extraction Control 670 cells contain a known concentration of internal control DNA and are spiked into the lysis buffer with the sample prior to DNA extraction. Following DNA extraction, the Control Mix is added to the extracted DNA prior to amplification (Fig. 1). Presence of internal control DNA confirms the success of the extraction step and reduces the chance of obtaining a false negative result in the sample DNA.
Bioline has specially developed a DNA Extraction Control which more closely mimics the test sample, as compared to spike controls. The genetic material from the test sample and the DEC is simultaneously extracted by common extraction methods, with the extraction control being as sensitive to inhibition and extraction failure as the test sample.
The Control Mix containing primers and Quasar® 670 labeled probe is used with DNA Extraction Control 670 and added to the extracted sample prior to amplification. Signal derived from the Internal Control DNA confirms the success of the extraction step (Fig. 2). DEC also monitors co-purification of PCR inhibitors (Fig. 3) that may cause biased or false amplification patterns.
All components should be stored at -20°C upon receipt. When stored under the recommended conditions and handled correctly, full activity is retained until the expiry date on the outer box label.
Shipped on dry/blue ice.