ISOLATE II FFPE RNA/DNA Kit

Cat No. Size List Price* Qty  
BIO-52087 50 Preps $585.00
 
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ISOLATE II FFPE RNA/DNA Kit is designed for the isolation of high yield, pure total RNA, including small RNA and genomic DNA from formalin-fixed paraffin embedded (FFPE) tissue samples.

Features & Benefits

  • Reliable - RNA/DNA from a single FFPE sample with no splitting of the lysate, reducing variability and inconsistent results
  • Isolate diverse RNA and DNA species - all sizes of RNA from large mRNA down to miRNA and all DNA above 80bp
  • Reversal of formalin crosslinks - optimized for high nucleic acid yield and purity
  • Phenol-free - all RNA including small RNA purified without bias
  • DNase I included - complete removal of genomic DNA from RNA fraction

Applications

The purified RNA can be used in a number of downstream applications including:
  • Real-Time RT-PCR
  • RNA-seq
  • Expression microarray
The purified genomic DNA can be used in:
  • Real-Time PCR
  • Multiplex PCR
  • Methylation analyses by bisulfite conversion and sequencing
  • Next generation sequencing
  • SNP microarray

Description

ISOLATE II FFPE RNA/DNA Kit is specially designed for the rapid, phenol-free, sequential isolation of pure total RNA (including small RNA) and genomic DNA from challenging FFPE tissue samples. Formalin fixation of tissues results in crosslinking of the nucleic acids and proteins. Furthermore, the embedding process can also lead to fragmented nucleic acids with time, often resulting in lower molecular weight nucleic acids than those obtained from fresh or frozen samples. ISOLATE II FFPE RNA/DNA Kit has been developed to allow for partial reversing of formalin modifications resulting in high quality and yield of nucleic acids.

The recovery of total RNA and genomic DNA from the same sample provides the distinct advantage of providing matched nucleic acid fractions, from the same population of cells. Matched RNA/DNA is invaluable for integrative studies of the genome, epigenome and transcriptome. Maximizing RNA and DNA retrieval from a single specimen is also crucial when using tissues that are of limited availability.

Isolation is based on a fast spin column format using a novel affinity resin as the separation matrix. The RNA and DNA are preferentially purified from other cellular components without the use of phenol or chloroform, resulting in a high quality and yield of nucleic acids. The ISOLATE II FFPE RNA/DNA Kit also overcomes the bias seen in recovery of the small RNA fraction observed with phenol-based RNA recovery.

The purified RNA can be used in a number of downstream applications including real-time RT-PCR, microarrays and RNA-seq. The purified genomic DNA can be used in real-time PCR, next-generation sequencing, multiplex PCR (fig. 1) and bisulfite conversion for epigenetic analysis.


Components

Reagent 50 Reactions
ISOLATE II FFPE RNA Micro Columns 50
ISOLATE II FFPE DNA Micro Columns 50
Collection Tubes (2ml) 100
Elution Tubes (1.7ml) 100
Proteinase K (lyophilized) 2 x 12mg
Digestion Buffer DX 2 x 25ml
Lysis Buffer RX 40ml
Wash Buffer W1 2 x 38ml
RNA Elution Buffer 6ml
DNA Elution Buffer 15ml
DNase I Solution (RNase-free) 210µl
DNase I Reaction Buffer DRB 6ml
Product Manual 1
Bench Protocol Sheet 1

Storage & Stability

The DNase I Solution and Proteinase K should be stored at -20°C upon arrival. All other components should be stored dry and at room temperature.

When stored under the recommended conditions and handled correctly, full activity of reagents is retained until the expiry date indicated on the outer box label.


Shipping conditions

Ambient temperature







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