ISOLATE II RNA/DNA/Protein Kit is a specially developed 3-in-1 kit that provides a rapid method for the sequential isolation and purification of high quality total RNA (including small RNA), genomic DNA and protein from a single sample of cultured cells, mammalian tissue, biofluids (including saliva, urine, semen and blood), bacteria, yeast, fungi or plants.
ISOLATE II RNA/DNA/Protein Kit is ideal for the isolation of macromolecules from precious or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis becomes more reliable since the RNA, DNA and proteins are derived from the same sample (i.e. purified molecules are from the same population of cells being studied).
ISOLATE II RNA/DNA/Protein Kit allows convenient processing of multiple samples in 30 minutes, utilizing a two-column system. Genomic DNA is bound to the first column while the total RNA and proteins are removed in the flow-through. The bound DNA is then washed and highly purified DNA is eluted. The flow-through is then loaded onto the second column, where total RNA is bound while the proteins are removed in the flow-through. The bound total RNA is washed and purified RNA is eluted (fig. 1 and 2). Proteins are rapidly purified and eluted using the column.
ISOLATE II RNA/DNA/Protein Kit allows for highly reliable integrative analysis of the transcriptome, genome and proteome, since the RNA, DNA and proteins are derived from the same sample and the same cell population. The purified nucleic acids and proteins are of the highest purity and integrity and can be used in a wide variety of downstream applications including expression profiling, miRNA profiling, siRNA gene silencing studies, novel biomarker discovery, epigenetics, genotyping, transgenic analysis and cell line characterization.
|ISOLATE II RNA/Protein Columns||50|
|ISOLATE II DNA Columns||50|
|Elution Tubes (1.7ml)||150|
|Elution Tubes (1.7ml)||150|
|Lysis Buffer TX||40ml|
|Wash Buffer W1 (Concentrate)||2 x 38ml|
|Wash Buffer W2 (Concentrate)||15ml|
|Wash Buffer W3||30ml|
|DNase I Reaction Buffer DRB||6ml|
|DNase I Solution (RNase-free)||0.8ml|
|RNA Elution Buffer||6ml|
|DNA Elution Buffer||6ml|
|Protein Elution Buffer||8ml|
|Protein Binding Buffer||8ml|
|Protein Neutralization Buffer||4ml|
|Protein Loading Dye||2ml|
|Bench Protocol Sheet||1|
The DNase I Solution should be stored at -20°C upon arrival. All other components should be stored dry and at room temperature.
When stored under the recommended conditions and handled correctly, full activity of reagents is retained until the expiry date indicated on the outer box label.
Certificates of Analysis for ISOLATE II RNA/DNA/Protein Kit are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.