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    Cat. No.
    Size
    List Price*
    QTY
    BIO-37084
    5ml
    $78.00
    +
    BIO-37085
    5 x 5ml
    $282.00
    +
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    Description

    Proteinase K is a highly active serine protease with broad cleavage specificity on native and denatured proteins. Proteinase K is widely used in the purification of native RNA and DNA from tissues or cell lines and many other applications.

    Product Highlights

    • High activity - broad-spectrum serine protease
    • Active under denaturing conditions - useful for a variety of applications
    • Stable - at high temperatures
    • Molecular biology grade - useful for general digestion of proteins
    • Proteinase K is also available in powder form

    Product Description


    Proteinase K is a highly active serine protease (MW 28,500 Da) isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of native RNA and DNA from tissues or cell lines. Because the solution is tested for the absence of RNases and DNases, it is especially suitable for isolating PCR and RT-PCR templates.

    The activity of Proteinase K is increased in the presence of denaturants such as SDS (1%) and at elevated temperature (50-60°C). The recommended working concentration is 50-100 µg/mL for protein removal and enzyme inactivation and up to 2 mg/mL for tissue treatment.

    Proteinase K products are free of detectable DNase and RNase.

    Applications

    • Inactivation of RNases/DNases during nucleic acid extraction
    • Protein modification
    • General protein digestion
    • Determination of enzyme localization
    • Remove nucleases for in situ hybridization
    • Improving cloning efficiency of PCR products

    Specification

    Components

    Reagent

    BIO-37084

     BIO-37085

    Proteinase K Solution (20mg/ml)

    5 mL

    5 x 5 mL

    Concentration


    Proteinase K is available as a powder or as a stabilized ready-to-use 20 mg/mL (600 mAU/mL) stock solution using a specially formulated calcium acetate buffer.

    Storage & Stability

    Proteinase K can be stored for 12 months at -20°C.

    Shipping conditions


    On Dry Ice or Blue Ice.