Tetro cDNA Synthesis Kit

Cat No. Size List Price* Qty  
BIO-65042 30 Reactions $149.50
BIO-65043 100 Reactions $469.00
*For more information on pricing please contact us

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The Tetro cDNA Synthesis Kit contains all the necessary components to generate cDNA from an RNA template. The generated cDNA is suitable for PCR with gene-specific primers or for other downstream applications. The kit contains MMLV Reverse Transcriptase and is suitable for first strand cDNA synthesis, cDNA library construction and the production of templates for PCR amplification.

Features & Benefits

  • Highly sensitive -for high-quality, full length cDNA from as little as 10pg of total RNA
  • Ultra-stable reverse transcriptase -for long genes and rare transcripts
  • Simple -one-tube, one-step set-up for highly sensitive PCR
  • Broad dynamic range -10pg to 2μg of RNA


  • Construction of cDNA libraries
  • 2-step PCR assays
  • Generation of probes for hybridization
  • Gene cloning


The Tetro cDNA Synthesis Kit contains our highly sensitive MMLV reverse transcriptase, oligo (dT)18 and random hexamer primers and all the necessary components to generate high quality cDNA from RNA templates (fig. 1). The first-strand cDNA generated is ideal for PCR (fig. 2) and can be used in a variety of other applications, such as  analyses of cellular RNAs, characterization of RNA splice variants, and the generation and cloning of cDNA. 

The Tetro cDNA Synthesis Kit is optimized for RT reactions over a wide range of total RNA concentrations (10pg-2μg), such that long and low-abundance cDNAs can be detected by amplification after cDNA synthesis. The kit contains oligo (dT)18 and random hexamer primers. The kit components are fully optimized to generate maximum yields of full-length cDNA.



30 reactions

100 reactions

5x RT Buffer



Reverse Transcriptase (200u/µl)



RNase Inhibitor (10u/µl)



dNTP Mix 10mM Total



Oligo (dT)18 Primer Mix



Random Hexamer Primer Mix



DEPC-treated Water



Storage & Stability

All kit components should be stored at -20°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.

Shipping conditions

Tetro cDNA Synthesis Kit is shipped on dry/blue ice

Customer Testimonials

Having used the Bioline Tetro cDNA Synthesis Kit and SensiFast SYBR and Probe mixes in our research for the past 2-3 years, I have no hesitation in recommending them. Besides offering great economy in purchase costs, the reagents perform with great accuracy, sensitivity and consistency in our assays which gives us great reproducibility in our results.
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We started to use this kit in our lab for all our routine cDNA synthesis and we haven't change since.
We find that the quality of the cDNA obtained is very good, the kit is very easy to use and it has a great quality-price ratio.
I definitely recommend it.

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