Alpha-Select Gold Efficiency

Cat No. Size List Price* Qty  
BIO-85027 1ml (20 x 50µl) $172.00
*For more information on pricing please contact us

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α-Select Gold Competent Cells are ideal for generating high efficiency libraries cloning with limited amounts of DNA.

Features & Benefits

  • Reduced recombination of cloned DNA - accommodates larger plasmids
  • High efficiency - available in ≥109 cfu/μg, perfect for library construction
  • Easy screening - with blue/white color selection
  • Convenient size - small aliquots reduce waste


  • Transformation of large plasmids into bacterial cells
  • Generating cDNA libraries
  • Blue/white color screening


α-Select Gold Competent Cells are directly comparable to DH5α cells. They contain a lacZ marker that provides α-complementation of the ß-galactosidase gene for blue/white color screening and are ideal for construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors.

α-Select Gold Competent Cells also provide recA1 and endA1 markers to minimize recombination and enhance the quality of the plasmid DNA, allowing efficient transformation even with large plasmids.

pUC19 DNA is also provided as a positive control.


Genotype: F- deoR endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) supE44 thi-1 phoA Δ(lacZYA-argF)U169 Φ80lacZΔM15 λ-




Chemically Competent Cells

20 x 50µl

pUC19 control vector (10pg/µl)


Storage & Stability

All components should be stored at -80°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

Shipping conditions

Shipped on dry ice.

Certificates of Analysis (COAs)

Certificates of Analysis for Alpha-Select Gold Efficiency are grouped by catalogue number and then listed by batch number. Larger pack sizes are listed first. To locate the COA for your product, first find the catalogue number and then the required batch number.

View all 29 COAs for Alpha-Select Gold Efficiency →

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