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Thank you, Tennille Sibbritt from the Children's Medical Research Institute - Embryology Unit, for a great review on our SensiFAST™ SYBR® No-ROX Kit.
"I am preparing samples for RNA-seq using a protocol that has been optimised for low input samples. I performed a qPCR on two housekeeping genes (Actb and Tbp) as a quality control step to ensure good amplification of these genes before proceeding to library preparation. I initially performed the qPCR using a combination of Taq and SYBR, which led to Ct values of 20-22 for Tbp and 23-26 for Actb. I repeated this qPCR using the 200 L sample of SensiFAST SYBR No ROX master mix I received. This worked very well and was quite sensitive. The Ct values for Tbp were 19-20 and for Actb were 17-19, while the no template control had minimal amplification. I plan on using SensiFAST SYBR No ROX master mix in the future, especially since it is substantially cheaper than what I was previously using."
Click here to learn more about our >> SensiFAST™ Real-Time PCR Kits
Kelvin Tuong, Research Officer at Ian Frazer Lab, The Translational Research Institute (TRI), UQDI... Reviews ISOLATE II RNA Micro Kit, telling you why he chose it and why he will continue to use Bioline products.
For more information on ISOLATE II RNA Micro Kit, click here http://www.bioline.com/isolate-ii-rna-micro-kit.html
Learn more about our ISOLATE II product range, click here http://www.bioline.com/isolate.html
Daisy and Magda, both PhD researchers from Lens Research Lab at the University of Sydney are introduced to PCR and the SensiFAST™ product range.
Review: "We used the ISOLATE II RNA Micro Kit, SensiFAST cDNA Synthesis Kit and SensiFAST SYBR® No-ROX Kit to perform our PCR and got excellent results. Our standard curves were great with r2 values over 0.99. Very impressed and pleased with this result, especially given it was our first time doing real-time PCR. Hope you like the video review we put together!"
To learn more about our SensiFAST range visit www.bioline.com/sensifast
Thank you, Sharon Pok from the Australian National University Medical School, for a great review on our ISOLATE II RNA Mini Kit.
"I recently used the ISOLATE II RNA Mini Kit. I had some cultured cells which are derived after FACS, which are not very many, to extract RNA from. Because I had a small starting quantity (<1E6 cells), I wasn’t expecting an extremely high yield. However, I attained a relatively good yield from that small amount of starting sample. What also impressed me was the 260/280 and 260/230 ratios. I have been having trouble getting a good 260/230 ratio lately, probably because of residual guanidine not being washed off properly. With the kit, both ratios were well above 2.0. Waiting to run the samples on the Bioanalyzer to obtain RIN values."
Click here to view Sharon's Results >> ISOLATE II RNA Mini Kit
Click here to learn more about our >> ISOLATE II Nucleic Acid Isolation family
Thank you, Joey Liu from Ohio State University, for a great review on our SensiFAST™ SYBR® No-ROX Kit.
"The performance of the SensiFAST™ SYBR® No-ROX Kit is robust. We have used it with ABI 7900 and Roche LightCycler 480 for gene expression for a while. The studies include a variety of human and mouse primary cells and cell lines. We are very happy with the kit because of its sensitivity and repeatability and will continue to go with it."
Click here to learn more about >> SensiFAST™ SYBR® No-ROX Kit
Thank you, Kwan Tzu Nin from The Institute for Marine and Antarctic Studies, for a great review on our MyTaq™ Extract-PCR Kit.
"MyTaq™ Extract-PCR Kit was effective and efficient for high throughput genotyping using as little as 1 mg of fish fin clip samples"
Click here to learn more about >> MyTaq™ Extract-PCR Kit