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MBE illumigene Malaria Milestone Infographic

By Bioline March 2, 2017
MBE Malaria Infographic



#illumigene #Malaria #Infographic #MeridianBioscience

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illumigene Malaria by Meridian Bioscience

By Bioline March 1, 2017

illumigene Malaria by Meridian Bioscience

The diagnosis of Malaria (Plasmodium sp.) has remained a critical piece in both the control of this vector borne disease and clinical case management of patients affected by malaria.

Traditional methods of diagnosis have long remained entrenched as the status quo. Methods such as microscopy and rapid diagnostic tests (RDTs) have continued to be used in both non-endemic and endemic settings. Molecular detection methods have emerged, but have so far fallen short of widespread adoption due to a number of key factors: bulky, specialist capital equipment requirements, expense, highly-trained staff with expertise, cold storage of reagents, complex workflows & impractical field use.

illumigene Malaria by Meridian Bioscience, www.meridianbioscience.eu in the space of 12 months has literally transformed molecular diagnosis offering an innovative choice of molecular diagnostic assays suited not only to non-endemic, Western European laboratories, handling imported malaria cases, but serving a crucial role in both the endemic regions of sub-Saharan Africa and the sub-microscopic, low parasitemia reservoirs of asymptomatic carriers - a known source of transmission. This latter group is key in the success or failure of any in-country elimination programme to achieve full eradication of malaria. An expert, namely, Professor Daouda Ndiaye, Dakar, Senegal has remarked in a Malaria World expert blog that “early data suggests it has the potential to become a new gold standard for molecular malaria diagnosis for case investigation, particularly in pre-elimination settings”. This new paradigm was formally recognized during the JIB-ACNBH (Journées Internationales de Biologie/Association des Colloques Nationaux des Biologistes 2016 Conference, Paris, France, where it received 1st prize in the Innovation category: emergency treatment and point-of-care testing.

illumigene, thus far, in the hands of a multitude of parasitology experts across Europe, Middle East and Africa, has repeatedly demonstrated its accuracy, with a proven negative predictive value (NPV) of 100% and equally it's performance in reliably screening for positives in less than 1 hour, achieving high levels of sensitivity and unparalleled ease-of-use.

During World Malaria Day, April 25, 2017, Dr Tom van Gool (Academic Medical Centre, Amsterdam, Netherlands) as part of a morning session on parasite diagnosis, will present ground breaking results from a multi-centric trial, following an evaluation of the illumigene Malaria assays. This scientific presentation will take place during ECCMID 2017 (The 27th European Congress of Clinical Microbiology and Infectious Diseases), www.eccmid2017.org hosted this year in Vienna, Austria; an event regarded by many as the leading flagship congress worldwide, for microbiologists and infectious disease specialists with a projected attendance of 15,000 participants and over 3,000 abstracts accepted as part of its broad scientific programme.

TESTIMONIALS:

"illumigene Malaria will establish itself as a milestone in Malaria diagnosis history, much like the cell counter-blood analyser has done following its introduction into the routine laboratory."

"illumigene will set a new paradigm in reliably mitigating against false positives, thereby facilitating a far easier diagnosis."

"In situations where ILG calls a positive result as a frontline screen ahead of a haemoscopy negative result for the same blood sample(s), then further investigations will be required to reconcile the result with the clinical picture. For this reason, the result can be considered esoteric with clinical expertise and laboratory input being required."

Romualo Grande, MD
UOC Microbiologia Clinica
Sacco Ospedale, ITALY


#illumigene #MeridianBioscience #Malaria

Bioline Scholar - Human Genes, Genetics, Genomes (Special ASHG 2012 Edition)

By Bioline: The PCR Company December 19, 2012 No comments

ASHG 2012 American Society of Human Genetics 2012 Annual Meeting

Bioline was once again an exhibitor (#Booth 1324-1326) at the American Society of Human Genetics (ASHG) annual meeting held in San Francisco in November. The largest human genetics meeting in the world, ASHG 2012 attracted over 7000 delegates to discuss the very latest advances in basic and clinical research.

Sessions included topics such as comparative epigenomics, early exome sequencing in complex traits, large-scale identification of regulators of translation and integrated genetic and functional studies of disease-causing variants.

Bioline USA at ASHG2012 Bioline USA at ASHG 2012

Bioline unveiled its new format exhibition stand at the conference, where our representatives met with scientists and discussed solutions to their molecular biology challenges. Bioline PCR Specialist, Dr. Sven Bocklandt gave a series of well received and thought-provoking talks on some of our latest products, including SensiFAST™, MyFi™ and MyTaq™ DNA polymerases. Exciting results from both published papers and some unpublished experiments from the Bioline scientific community were presented, including results from the Bioline PCR Challenge.

In keeping with ASHG 2012, this month's Bioline scholar is dedicated to Human Genetics. We are pleased to highlight a selection of recent papers featuring Bioline products, perfectly suited for both small- and large-scale genetic and genomic studies in humans.

BIO-X-ACT™ Long DNA Polymerase

Mutations in MTM1 (myotubularin 1) cause X-linked myotubular myopathy that affects 1 in 50,000 males. In neonates or infants, movement and breathing are severely affected. The authors developed a MTM1-specific database of genetic variants, with 474 identified mutations from 472 patients. Next-Gen sequencing (Illumina HiSeq) and cDNA analysis revealed the presence of a novel MTM1/MAMLD1 fusion transcript in one case.

Oliveira, J. et al. (2012). Expanding the MTM1 mutational spectrum: novel variants including the first multi-exonic duplication and development of a locus-specific database. Eur. J. Hum. Genet. doi: 10.1038/ejhg.2012.201

cDNA synthesis kit

Children with X-linked retinitis pigmentosa (XLRP) often go blind by the second decade of life. A severe form of XLRP, RP23, was previously mapped to a 10.71Mb interval on Xp22.31-22.13, containing 62 genes. Using targeted NGS (Illumina), the authors identified a deep intronic mutation in OFD1 as the most likely cause of RP23. Insertion of a cryptic exon produces an aberrant transcript and reduced levels of correctly spliced transcript. This is predicted to result in a severely truncated protein and decreased levels of normal protein.

Webb, T.R. et al. (2012). Deep intronic mutation in OFD1, identified by targeted genomic next-generation sequencing, causes a severe form of X-linked retinitis pigmentosa (RP23). Hum. Mol. Genet. 21(16):3647-3654.

BIOLASE™ DNA Polymerase

The genetic basis for susceptibility to malaria in a Vietnamese population was investigated by SNP genotyping by researchers from Oxford University and the MalariaGen consortium. Data from 65 SNPs in 42 malarial candidate genes in 956 severe malaria cases and 2350 controls from Vietnam was reported. Variants in six genes (ICAM1, IL1A, IL17RC, IL13, LTA and TNF) encoding adhesion and pro-inflammatory molecules were associated with severe malaria.

Dunstan, S.J. et al. (2012). Variation in human genes encoding adhesion and proinflammatory molecules are associated with severe malaria in the Vietnamese. Genes Immun. 13(6):503-508.

BioMix™ Red

Facioscapulohumeral muscular dystrophy (FSHD), characterized by muscle weakness and wasting, is associated with a shortened telomeric chr 4q35 due to deletions of the D4Z4 tandem repeat (FSHD1) or DNA methylation changes of D4Z4 (FSHD2). Using exome sequencing (Illumina NGS) the authors identified two known pathogenic mutations in CAPN3, indicating a case of limb-girdle muscular dystrophy type 2A (LGMD2A) rather than FSHD2. The authors conclude that ‘diagnosis by sequencing’ of FSHD may be more commonly adopted in clinical genetics laboratories around the world.

Leidenroth, A. et al. (2012). Diagnosis by sequencing: correction of misdiagnosis from FSHD2 to LGMD2A by whole-exome analysis. Eur. J. Hum. Genet. 20(9):999-1003

An arrayed human genomic library comprising 115,000 PAC clones was constructed. Functional studies with a p53-containing PAC clone in p53-null human osteosarcoma cells showed the utility of individual library members in human cell culture models. The library can be used to validate candidate genes identified by GWAS and for gene therapy in different recessive disorders.

Fuesler, J. et al. (2012). An arrayed human genomic library constructed in the PAC shuttle vector pJCPAC-Mam2 for genome-wide association studies and gene therapy. Gene. 496(2):103-109

Immomix™

Charcot-Marie-Tooth disease (CMT) is an inherited disorder of peripheral nerve dysfunction resulting in numbness and weakness. Mutations in four genes encoding an aminoacyl-tRNA synthetase (ARS) have been associated with CMT. This study from the University of Michigan found that the p.Arg329 AARS mutation is a recurrent, loss-of-function mutation that arises due to methylation-mediated deamination of a CpG dinucleotide.

McLaughlin, H.M. et al. (2012). A Recurrent loss-of-function alanyl-tRNA synthetase (AARS) mutation in patients with charcot-marie-tooth disease type 2N (CMT2N) Hum. Mutat. 33(1):244-253

Introducing the Bioline Scholar Publications Database

Introducing Bioline Scholar - from Bioline: The PCR Company Bioline Scholar - from Bioline: The PCR Company

If you're interested in knowing more about the fields and areas of research in which Bioline products have been used to further knowledge, we've added a Bioline Scholar page to our web site listing the papers and publications in which our molecular biology reagents are featured.

You can search for publications by author, title, topic and product and the archive contains papers from 1994 through to the present.  Currently the Bioline Scholar database lists and provides links to publications where the research was conducted using the following Bioline products:

That's it for the Bioline blog in 2012—season's greetings to all our customers and readers alike, from all at Bioline—see you in 2013!

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